Citation
Ang B. H., . and Chan L. K., . (2000) Effects of N6-Benzyladenine BA on in vitro culture of Spilanthes acmella. [Proceedings Paper]
Abstract
The effects of N6-benzyladenine BA on multiple shoot formation of Spilanthes acmella were studied. Axillary buds of the plant were used as explants which were surface sterilised using mercuric chloride HgC12 0.08 w/v followed by 15 Clorox for 5 and 15 minutes respectively. The aseptic explants were cultured on Murashige and Skoog MS medium supplemented with BA 0.0 2 4 6 8.0 and 10.0 mg/l for induction of multiple shoots over a period of five weeks. Multiple shoots were formed in these media supplemented with 2.0 4 6.0 and 8.0 mg/l of BA. Results indicated that the addition of 6 mg/l of BA or more caused retardation and swelling of explants which eventually resulted in abnormal structures and death. The number of shoots formed after sub-culturing in the fifth week was found to be higher in those explants undergoing shoot separation as compared to non-separated individual shoots. MS medium supplemented with BA of 2 mg/l or less was the most suitable for the production of multiple shoots of S. acmella.
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Abstract
The effects of N6-benzyladenine BA on multiple shoot formation of Spilanthes acmella were studied. Axillary buds of the plant were used as explants which were surface sterilised using mercuric chloride HgC12 0.08 w/v followed by 15 Clorox for 5 and 15 minutes respectively. The aseptic explants were cultured on Murashige and Skoog MS medium supplemented with BA 0.0 2 4 6 8.0 and 10.0 mg/l for induction of multiple shoots over a period of five weeks. Multiple shoots were formed in these media supplemented with 2.0 4 6.0 and 8.0 mg/l of BA. Results indicated that the addition of 6 mg/l of BA or more caused retardation and swelling of explants which eventually resulted in abnormal structures and death. The number of shoots formed after sub-culturing in the fifth week was found to be higher in those explants undergoing shoot separation as compared to non-separated individual shoots. MS medium supplemented with BA of 2 mg/l or less was the most suitable for the production of multiple shoots of S. acmella.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal QK99 A1S471 2000 Call Number. |
| AGROVOC Term: | SPILANTHES |
| AGROVOC Term: | BA |
| AGROVOC Term: | IN VITRO CULTURE |
| AGROVOC Term: | EXPLANTS |
| AGROVOC Term: | MALAYSIA |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:13 |
| URI: | http://webagris.upm.edu.my/id/eprint/11309 |
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