Citation
Chan L. K., . and Lee W. L., . (2000) Micropropagation of Orthosiphon stamineus. [Proceedings Paper]
Abstract
A micropropagation method for Orthosiphon stamineus a traditional medicinal plant has been developed. Nodal segments from in vitro growing shoots were used as explants. Explants were cultured on Murashige and Skoog MS medium supplemented with various combination of benzyladenine BA and indole-3-butyric acid IBA 30 g/l sucrose and 7 g/l agar. The highest shoot poliferation rates were obtained on MS supplemented with 1.5 mg/l BA with an average of sixteen 15.8 shoots per explant over a period of six weeks. Rooting occurred when in vitro shoots were cultured in basic MS medium or with the addition of 6 mg/l of IBA. An average of six roots 5.6 per shoot were produced when cultured in MS supplemented with 6 mg/l IBA for two weeks. All the in vitro plantlets survived when transferred to the external environment.
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Abstract
A micropropagation method for Orthosiphon stamineus a traditional medicinal plant has been developed. Nodal segments from in vitro growing shoots were used as explants. Explants were cultured on Murashige and Skoog MS medium supplemented with various combination of benzyladenine BA and indole-3-butyric acid IBA 30 g/l sucrose and 7 g/l agar. The highest shoot poliferation rates were obtained on MS supplemented with 1.5 mg/l BA with an average of sixteen 15.8 shoots per explant over a period of six weeks. Rooting occurred when in vitro shoots were cultured in basic MS medium or with the addition of 6 mg/l of IBA. An average of six roots 5.6 per shoot were produced when cultured in MS supplemented with 6 mg/l IBA for two weeks. All the in vitro plantlets survived when transferred to the external environment.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal QK99 A1S471 2000 Call Number. |
| AGROVOC Term: | MICROPROPAGATION |
| AGROVOC Term: | IN VITRO |
| AGROVOC Term: | EXPLANTS |
| AGROVOC Term: | SEEDLINGS |
| AGROVOC Term: | MALAYSIA |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:13 |
| URI: | http://webagris.upm.edu.my/id/eprint/11310 |
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