Citation
Boey P. L., . and Abu Hassan A., . and Chan L. K., . and Ku Nornadia K. R., . and Tran VM., . (2010) Somatic embryogenesis of Artemisia Annua L. [Proceedings Paper]
Abstract
Artemisia annua L. is one of the most important medicinal plants in the world for its artemisinin an anti-malarial drug. Commercially available artemisinin is mainly extracted from the wild plants hence subjected to seasonal and other environmental limitations. Somatic embryogenesis an in vitro culture technology provides an alternative to overcome these limitations. Somatic embryos could be induced from the leaf-derived callus of A. annua. Callus was initiated from the leaf pieces on callus induction medium Murashige and Skoog MS medium supplemented with 0.5 mg/I naphathaleneacetic acid NAA and 0.5 mg/I 6- benzylaminopurine BA 0.5 g casein hyrolysate and 30 g/l sucrose. Optimum callus production and variations in dallus morphology colour and texture were observed and evaluated. After one week of transferring the callus tissues onto basic MS medium without any plant growth regulator the callus that were pre-treated with various concentration of 2 4-dichlorophenoxyacetic acid formed somatic embryos. Histological studies confirmed the formation of somatic embryos. Somatic embryos that contained artemisinin will be tested against mosquito larvae in subsequent study.
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Abstract
Artemisia annua L. is one of the most important medicinal plants in the world for its artemisinin an anti-malarial drug. Commercially available artemisinin is mainly extracted from the wild plants hence subjected to seasonal and other environmental limitations. Somatic embryogenesis an in vitro culture technology provides an alternative to overcome these limitations. Somatic embryos could be induced from the leaf-derived callus of A. annua. Callus was initiated from the leaf pieces on callus induction medium Murashige and Skoog MS medium supplemented with 0.5 mg/I naphathaleneacetic acid NAA and 0.5 mg/I 6- benzylaminopurine BA 0.5 g casein hyrolysate and 30 g/l sucrose. Optimum callus production and variations in dallus morphology colour and texture were observed and evaluated. After one week of transferring the callus tissues onto basic MS medium without any plant growth regulator the callus that were pre-treated with various concentration of 2 4-dichlorophenoxyacetic acid formed somatic embryos. Histological studies confirmed the formation of somatic embryos. Somatic embryos that contained artemisinin will be tested against mosquito larvae in subsequent study.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | 5 ref. SB 293 S471 2010 |
| AGROVOC Term: | Somatic embryogenesis |
| AGROVOC Term: | Artemisia annua |
| AGROVOC Term: | Medicinal plants |
| AGROVOC Term: | Artemisinin |
| AGROVOC Term: | Callus culture |
| AGROVOC Term: | Asteraceae |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:14 |
| URI: | http://webagris.upm.edu.my/id/eprint/11546 |
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