Micropropagation of Artemisia annua L. and the effects of in vitro culture conditions on the morphology of leaf stomata and trichomes


Citation

Nur Izzati A. H., . and Chan L. K., . and Goh Y. Z., . and Bhatt A., . (2010) Micropropagation of Artemisia annua L. and the effects of in vitro culture conditions on the morphology of leaf stomata and trichomes. [Proceedings Paper]

Abstract

Artemisia annua L. has traditionally been used in China as herbal medicine for the treatment of various ailments. Since the discovery of artemisinin from this plant as an anti-malarial agent it has been cultivated in many countries in Europe America Africa as well as Asia. It grows very well mainly in high altitude regions or at areas with a pronounced cool period. Due to the demand of artemisinin as an anti-malarial drug especially against drug resistant malaria good elite clones were sought after to reduce the variation of field grown plants derived from seeds. A micropropagation protocol has been successfully established for mass production of A. annua clones. However the in vitro condition was found to affect the structures and distribution of the leaf trichomes an important organ for the production of artemisinin. This study reports the mass production of A. annua plantlets using nodal segments as the explants. The acclimatized plants placed in the green house with tropical type of condition was found to affect the artemisinin content which was lower than those planted in a controlled cool environment.


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Abstract

Artemisia annua L. has traditionally been used in China as herbal medicine for the treatment of various ailments. Since the discovery of artemisinin from this plant as an anti-malarial agent it has been cultivated in many countries in Europe America Africa as well as Asia. It grows very well mainly in high altitude regions or at areas with a pronounced cool period. Due to the demand of artemisinin as an anti-malarial drug especially against drug resistant malaria good elite clones were sought after to reduce the variation of field grown plants derived from seeds. A micropropagation protocol has been successfully established for mass production of A. annua clones. However the in vitro condition was found to affect the structures and distribution of the leaf trichomes an important organ for the production of artemisinin. This study reports the mass production of A. annua plantlets using nodal segments as the explants. The acclimatized plants placed in the green house with tropical type of condition was found to affect the artemisinin content which was lower than those planted in a controlled cool environment.

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: 5 ref. SB 293 S471 2010
AGROVOC Term: Acclimatization
AGROVOC Term: Artemisinin
AGROVOC Term: Micropropagation
AGROVOC Term: Trichomes
AGROVOC Term: Artemisia annua
AGROVOC Term: In vitro culture
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:14
URI: http://webagris.upm.edu.my/id/eprint/11547

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