Citation
Boey P. L., . and Sreeramanan S., . and Chan L. K., . and Lo K. Y., . and Tran V. M., . (2010) Comparative study on culture media for the establishment of cell suspension culture of Artemisia annua L. [Proceedings Paper]
Abstract
Artemisinin isolated from the aerial parts of Artemisia annua plants have been proven to be effective forthe treatment of both drug resistant and cerebral malaria parasites. Although the production of artemisinin by biological and chemical means is possible it is not economically feasible. Therefore enhanced production of artemisinin via in vitro cell culture technique is highly desirable. Friable leaf-derived callus of A. annua was transferred from the solid medium into nine different liquid media containing different chemical compositions to determine the suitable medium for the preparation of cell suspension culture of A. annua. Only two media namely the MS medium supplemented with 0.5mg/L 2 4-0 and MS medium added with 0.5 mg/L BA 0.5mg/L NAA and 0.5g/L casein hydrolysate were suitable for A. annua cells growth. Cells cultured in these two media were light green and green in colour respectively. After eight subcultures cycles of 14 days interval cells cultured in MS medium supplemented with 0.5mg/L 2 4-0 turned blackish brown and the cell growth index decreased after each subculture cycle indicated occurrence of cell death. Whereas cells cultured in MS medium 0.5 mg/L BA 0.5mg/L NAA 0.5g/L casein hydrolysate were green in colour and produced consistent cell biomass with growth index of 11.2 - 11.8 at each subculture cycle.
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Abstract
Artemisinin isolated from the aerial parts of Artemisia annua plants have been proven to be effective forthe treatment of both drug resistant and cerebral malaria parasites. Although the production of artemisinin by biological and chemical means is possible it is not economically feasible. Therefore enhanced production of artemisinin via in vitro cell culture technique is highly desirable. Friable leaf-derived callus of A. annua was transferred from the solid medium into nine different liquid media containing different chemical compositions to determine the suitable medium for the preparation of cell suspension culture of A. annua. Only two media namely the MS medium supplemented with 0.5mg/L 2 4-0 and MS medium added with 0.5 mg/L BA 0.5mg/L NAA and 0.5g/L casein hydrolysate were suitable for A. annua cells growth. Cells cultured in these two media were light green and green in colour respectively. After eight subcultures cycles of 14 days interval cells cultured in MS medium supplemented with 0.5mg/L 2 4-0 turned blackish brown and the cell growth index decreased after each subculture cycle indicated occurrence of cell death. Whereas cells cultured in MS medium 0.5 mg/L BA 0.5mg/L NAA 0.5g/L casein hydrolysate were green in colour and produced consistent cell biomass with growth index of 11.2 - 11.8 at each subculture cycle.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | 7 ref. SB 293 S471 2010 |
| AGROVOC Term: | Culture media |
| AGROVOC Term: | Artemisinin |
| AGROVOC Term: | Asteraceae |
| AGROVOC Term: | Suspension culture |
| AGROVOC Term: | Cell culture |
| AGROVOC Term: | Artemisia annua |
| AGROVOC Term: | Drug resistance |
| AGROVOC Term: | Cerebral malaria |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:14 |
| URI: | http://webagris.upm.edu.my/id/eprint/11549 |
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