Isolation and characterization of cellulolytic nitrogen-fixing and phosphate-solubilizing soil bacteria from a rehabilitated forest in Bintulu

Tang A., . and Wong S.K., . and O.H. Ahmed, . and Nik M. Majid, . (2011) Isolation and characterization of cellulolytic nitrogen-fixing and phosphate-solubilizing soil bacteria from a rehabilitated forest in Bintulu. [Proceedings Paper]

Studies of cellulolytic nitrogen-fixing and phosphate-solubilizing microorganisms composition are important especially in degraded soil of rehabilitated forests considering their major roles in elemental cycle and plant nutrition regulation. The objectives of this study were to: i isolate and characterize soil cellulolytic phosphate-solubilizing and nitrogen-fixing bacteria from a rehabilitated forest and ii identify selected cellulolytic phosphatesolubilizing and nitrogen-fixing bacteria. This study was conducted in a rehabilitated forest at Universiti Putra Malaysia Bintulu Sarawak Campus. Cellulolytic nitrogen-fixing and phosphate-solubilizing microorganisms were isolated on Congo red cellulose Nfb and PSM agar media respectively. The activities of the bacterial isolates were measured through index values based on the ratio of halo or clearing zone and colony diameters. Isolates with the highest activities were identified based on the 16SrRNA gene. Five isolates with high cellulose hydrolyzing index values identified as Serratia sp. Stenotrophomonas sp. and Bacillus thuringiensis while isolates with high phosphate-solubilization index values were closely related to be Burkholderia sp. and Gluconacetobacter diazotrophicus. Isolates with high nitrogen-fixation index were closely matched Burkholderia mimosarum Delftia tsuruhatensis Ralstonia sp. Cupriavidus basilensis and Stenotrophomonas sp. These cellulolytic nitrogen-fixing and phosphate-solubilizing bacterial isolated with high respective functional activities may serve as potential components of biological fertilizer for rehabilitated forests or crops.

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Abstract

Studies of cellulolytic nitrogen-fixing and phosphate-solubilizing microorganisms composition are important especially in degraded soil of rehabilitated forests considering their major roles in elemental cycle and plant nutrition regulation. The objectives of this study were to: i isolate and characterize soil cellulolytic phosphate-solubilizing and nitrogen-fixing bacteria from a rehabilitated forest and ii identify selected cellulolytic phosphatesolubilizing and nitrogen-fixing bacteria. This study was conducted in a rehabilitated forest at Universiti Putra Malaysia Bintulu Sarawak Campus. Cellulolytic nitrogen-fixing and phosphate-solubilizing microorganisms were isolated on Congo red cellulose Nfb and PSM agar media respectively. The activities of the bacterial isolates were measured through index values based on the ratio of halo or clearing zone and colony diameters. Isolates with the highest activities were identified based on the 16SrRNA gene. Five isolates with high cellulose hydrolyzing index values identified as Serratia sp. Stenotrophomonas sp. and Bacillus thuringiensis while isolates with high phosphate-solubilization index values were closely related to be Burkholderia sp. and Gluconacetobacter diazotrophicus. Isolates with high nitrogen-fixation index were closely matched Burkholderia mimosarum Delftia tsuruhatensis Ralstonia sp. Cupriavidus basilensis and Stenotrophomonas sp. These cellulolytic nitrogen-fixing and phosphate-solubilizing bacterial isolated with high respective functional activities may serve as potential components of biological fertilizer for rehabilitated forests or crops.

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Item Type:	Proceedings Paper
Additional Information:	9 ref. QH 541.5 R27 I61 2011
AGROVOC Term:	Cellulolytic microorganisms
AGROVOC Term:	Nitrogen
AGROVOC Term:	Phosphates
AGROVOC Term:	Soil
AGROVOC Term:	Bacteria
AGROVOC Term:	Malaysia
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/11596

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