Phytochemical and pharmacological investigations of Egyptian clover Trifolium alexandrinum L.

Ahmed Qamar U., . and Kaleem M., . and Parveen N., . and Khan N.U., . (2006) Phytochemical and pharmacological investigations of Egyptian clover Trifolium alexandrinum L. [Proceedings Paper]

In continuation of our studies on the phytoconstituents of the Trifolium species Fabaceae-Trifolieae mostly occurring in India and other parts of the world we have examined Trifolium alexandrinum L. which is also known as Barseem or Egyptian clover. Its aerial part is used as hay or pasture and seeds are used as an antidiabetic. Phytochemical data obtained with several Trifolium species previously investigated allowed the characterization of many secondary metabolites mainly consisting of flavonoids and aliphatic compounds structural types. The aim was to identify and characterize phenolic components in the leaves of T. alexandrinum for antimicrobial and antioxidant activities. Eight different flavonoids were detected after multistep chromatographic fractionation and preparative TLC using different solvent systems. The isolated flavonoids after purification from ethyl acetate extract were identified as 1 5 7-dihydroxy-4;-O-methyl isoflavone 2 5 7 4;trihydroxy isoflavone 3 5 7 4;-trihydroxy flavone 4 5 7 3; 4;-tetrahydroxy flavone 5 apigenin 7-0--D-galactopyranoside and 6 luteolin 7-0--glucoside by UV IR MS IH-NMR 13C-NMR analyses direct comparison with their authentic samples and different chemical methods. Occurrence of monoflavonoids 3 4 5 6 from this plant is being reported for the first time. Further studies are still in progress to elucidate the structure of the remaining two phenolic compounds. The inhibitory potencies and capacities of different extracts against many gram positive and gram negative bacteria were also tested.

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Abstract

In continuation of our studies on the phytoconstituents of the Trifolium species Fabaceae-Trifolieae mostly occurring in India and other parts of the world we have examined Trifolium alexandrinum L. which is also known as Barseem or Egyptian clover. Its aerial part is used as hay or pasture and seeds are used as an antidiabetic. Phytochemical data obtained with several Trifolium species previously investigated allowed the characterization of many secondary metabolites mainly consisting of flavonoids and aliphatic compounds structural types. The aim was to identify and characterize phenolic components in the leaves of T. alexandrinum for antimicrobial and antioxidant activities. Eight different flavonoids were detected after multistep chromatographic fractionation and preparative TLC using different solvent systems. The isolated flavonoids after purification from ethyl acetate extract were identified as 1 5 7-dihydroxy-4;-O-methyl isoflavone 2 5 7 4;trihydroxy isoflavone 3 5 7 4;-trihydroxy flavone 4 5 7 3; 4;-tetrahydroxy flavone 5 apigenin 7-0--D-galactopyranoside and 6 luteolin 7-0--glucoside by UV IR MS IH-NMR 13C-NMR analyses direct comparison with their authentic samples and different chemical methods. Occurrence of monoflavonoids 3 4 5 6 from this plant is being reported for the first time. Further studies are still in progress to elucidate the structure of the remaining two phenolic compounds. The inhibitory potencies and capacities of different extracts against many gram positive and gram negative bacteria were also tested.

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Item Type:	Proceedings Paper
Additional Information:	Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. RS180 M3I61 2005 Call Number
AGROVOC Term:	Phytochemistry
AGROVOC Term:	Pharmacology
AGROVOC Term:	Egyptian clover
AGROVOC Term:	Trifolium alexandrinum
AGROVOC Term:	Fabaceae
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/11714

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