Enzymatic hydrolysis and isolation of oil palm frond derived xylooligosaccharides by xylanase Trichoderma Viride

Farah Salina H., . and Fazilah A., . and Mohd Azemi M. N., . and Norziah M. H., . (2008) Enzymatic hydrolysis and isolation of oil palm frond derived xylooligosaccharides by xylanase Trichoderma Viride. [Proceedings Paper]

The isolation of xylooligosaccharides from delignified hemicellulose was conducted by using enzymatic hydrolysis method. The lignin content of OPF was removed by alkaline peroxide treatment at 80C and the extraction of hemicellulose was conducted using alkaline extraction method prior to treatment with enzyme xylanase Trichoderma Viride TV for 1-10 hours of incubation time. The optimum yields of xylooligosaccharides were obtained by comparing the enzyme concentrations 0.01 0.0005 0.001 0.002 0.3 0.4 w/v with 10 w/v substrate at temperature 50C and pH 5.5. The yields xylooligosaccharides xylotriose and xylobiose as well as xylose of each interval time were determined by using HPLC method. The results showed that the optimum yields were significantly obtained at 7 hours of incubation time with 0.3 w/v enzyme concentration yielding 2.42300.2400g/kg of xylotriose 3.34390.0745g/kg of xylobiose and 0.5500 0.01 36g/kg of xylose. By using the optimum conditions obtained the effect between crude and delignified substrate was also studied. The results showed that enzymatic hydrolysis on crude substrate yielded lower yield of xylooligosaccharides with 1.1607 0.0626 g/kg of xylotriose 0.8017 0.0047 g/kg of xylobiose and 0.5496 0.0136 g/kg of xylose respectively as compared to delignified substrate.

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Abstract

The isolation of xylooligosaccharides from delignified hemicellulose was conducted by using enzymatic hydrolysis method. The lignin content of OPF was removed by alkaline peroxide treatment at 80C and the extraction of hemicellulose was conducted using alkaline extraction method prior to treatment with enzyme xylanase Trichoderma Viride TV for 1-10 hours of incubation time. The optimum yields of xylooligosaccharides were obtained by comparing the enzyme concentrations 0.01 0.0005 0.001 0.002 0.3 0.4 w/v with 10 w/v substrate at temperature 50C and pH 5.5. The yields xylooligosaccharides xylotriose and xylobiose as well as xylose of each interval time were determined by using HPLC method. The results showed that the optimum yields were significantly obtained at 7 hours of incubation time with 0.3 w/v enzyme concentration yielding 2.42300.2400g/kg of xylotriose 3.34390.0745g/kg of xylobiose and 0.5500 0.01 36g/kg of xylose. By using the optimum conditions obtained the effect between crude and delignified substrate was also studied. The results showed that enzymatic hydrolysis on crude substrate yielded lower yield of xylooligosaccharides with 1.1607 0.0626 g/kg of xylotriose 0.8017 0.0047 g/kg of xylobiose and 0.5496 0.0136 g/kg of xylose respectively as compared to delignified substrate.

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Item Type:	Proceedings Paper
Additional Information:	Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. GE 90 M3 I61 2008 Call Number
AGROVOC Term:	Enzymatic hydrolysis
AGROVOC Term:	Isolation
AGROVOC Term:	Oil palm
AGROVOC Term:	Trichoderma viride
AGROVOC Term:	Hemicellulose
AGROVOC Term:	Hydrolysis
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/11783

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