The genetic diversity of the American oil palm Elaeis oleifera kunth Cortes revealed by nuclear RFLP markers

Barcelos E., . and Amblard P., . and Berthaud J., . and Segiun M., . (2006) The genetic diversity of the American oil palm Elaeis oleifera kunth Cortes revealed by nuclear RFLP markers. [Proceedings Paper]

The Restriction Fragment Length Polymorphism RFLP technique was used to assess the genetic diversity its organization and genetic relationships among 36 American oil palm Elaeis oleifera Kunth Cortes populations from Brazil Peru French Guyana Surinam north Colombia and Central America covering a large part of the natural distribution of the species. Polymorphism in 241 accessions was revealed with 37 nuclear cDNA probes and submitted to Factorial Analysis of Correspondences FA C cluster analysis and population genetics analysis Fstatistics. The genetic diversity of E. oleifera is strongly structured by geographical origin with four groups clearly distinguished: Brazil Surinam/French Guyana. North Colombia/Central America and Peru. Within the Amazon basin there is a moderate structure that corresponds to the major tributaries of the Amazon river. From the 37 polymorphic RFLP probe/enzyme combinations used 19 probes 51 presented simple restriction profiles with one or two bands per plant suggesting a single locus with different alleles allowing allelic co-dominant coding for them. This led to the identification of 59 alleles for all 19 loci. Three rare alleles. with frequencies lower than 5 were present in the Brazillian populations while no other allele was found to be rare or very frequent 95 in the whole species nor in any ofthe sub-geographic groups. Very low levels of polymorphism and heterozygosity were observed in the non-Brazilian groups Surinam/French Guyana north Colombia/Central America and Peru with some ofthese being even monomorphic and completely homozygous for the studied RFLP locus. A high genetic diversity was detected in E. oleifera by RFLP nuclear analysis with a mean of3.1 alleles in the 19 loci and H Nei 0.404. These values are higher than the genetic diversity estimated for the African E. guineensis by using the same probes over 51 accessions covering a broad area of the distribution of this species and are higher also than in other perennial species studied elsewhere.

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Abstract

The Restriction Fragment Length Polymorphism RFLP technique was used to assess the genetic diversity its organization and genetic relationships among 36 American oil palm Elaeis oleifera Kunth Cortes populations from Brazil Peru French Guyana Surinam north Colombia and Central America covering a large part of the natural distribution of the species. Polymorphism in 241 accessions was revealed with 37 nuclear cDNA probes and submitted to Factorial Analysis of Correspondences FA C cluster analysis and population genetics analysis Fstatistics. The genetic diversity of E. oleifera is strongly structured by geographical origin with four groups clearly distinguished: Brazil Surinam/French Guyana. North Colombia/Central America and Peru. Within the Amazon basin there is a moderate structure that corresponds to the major tributaries of the Amazon river. From the 37 polymorphic RFLP probe/enzyme combinations used 19 probes 51 presented simple restriction profiles with one or two bands per plant suggesting a single locus with different alleles allowing allelic co-dominant coding for them. This led to the identification of 59 alleles for all 19 loci. Three rare alleles. with frequencies lower than 5 were present in the Brazillian populations while no other allele was found to be rare or very frequent 95 in the whole species nor in any ofthe sub-geographic groups. Very low levels of polymorphism and heterozygosity were observed in the non-Brazilian groups Surinam/French Guyana north Colombia/Central America and Peru with some ofthese being even monomorphic and completely homozygous for the studied RFLP locus. A high genetic diversity was detected in E. oleifera by RFLP nuclear analysis with a mean of3.1 alleles in the 19 loci and H Nei 0.404. These values are higher than the genetic diversity estimated for the African E. guineensis by using the same probes over 51 accessions covering a broad area of the distribution of this species and are higher also than in other perennial species studied elsewhere.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. SB299 P3I61 2000 Call Number
AGROVOC Term:	Oil palm
AGROVOC Term:	Elaeis oleifera
AGROVOC Term:	Genetic diversity as resource
AGROVOC Term:	RFLP
AGROVOC Term:	Polymorphism
AGROVOC Term:	Plant breeders
AGROVOC Term:	DNA probes
AGROVOC Term:	Polymorphism
AGROVOC Term:	Isoenzymes
AGROVOC Term:	Germplasm
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/11894

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