Evaluation of lactic acid production via Lactobacillus rhamnosus fermentation from kenaf biomass using statistical design


Citation

Rashidi O., . and Maizirwan M., . and Nur Aimi M. N., . and Nurhafizah Seeni M., . and Hazleen A., . (2014) Evaluation of lactic acid production via Lactobacillus rhamnosus fermentation from kenaf biomass using statistical design. [Proceedings Paper]

Abstract

Recently many industries are aim to reduce the usage of petroleum based product or synthetic fibre due to the increment of the environmental consciousness. This has led to the extensive research on the biodegradable polymers from the natural fibre such as kenaf in order to produce eco -friendly product that will replaced the existing petroleum based products. The possible monomer in this matter is lactic acid which scientifically known as 2hydroxy propanoic acid. Lactic acid is a carboxylic acid and it is commercially produced from the food sources such as com and potato. The raw materials used in the lactic acid production are considered less economically since it has to compete with the food sources. Thus natural fibre such as kenaf is found the best alternative to substitute the raw material for lactic acid production. Since kenaf is one type of plant fibre it has a complex structure which naturally resistant to breakdown its structural sugar that will inhibit microorganisms to access through. Therefore chemical treatment is needed for the liberation of glucose which later will used as a substrate in fermentation process. Fermentation of lactic acid was carried out using Lactobacillus rhamnosus as the lactic acid microbes and this process was designed using Central Composite Design CCD from Design Expert v6.0.8 software. The highest yield oflactic acid which is 7.02 giL was occurred at run 5 when the value of peptone and yeast extract is at highest.


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Abstract

Recently many industries are aim to reduce the usage of petroleum based product or synthetic fibre due to the increment of the environmental consciousness. This has led to the extensive research on the biodegradable polymers from the natural fibre such as kenaf in order to produce eco -friendly product that will replaced the existing petroleum based products. The possible monomer in this matter is lactic acid which scientifically known as 2hydroxy propanoic acid. Lactic acid is a carboxylic acid and it is commercially produced from the food sources such as com and potato. The raw materials used in the lactic acid production are considered less economically since it has to compete with the food sources. Thus natural fibre such as kenaf is found the best alternative to substitute the raw material for lactic acid production. Since kenaf is one type of plant fibre it has a complex structure which naturally resistant to breakdown its structural sugar that will inhibit microorganisms to access through. Therefore chemical treatment is needed for the liberation of glucose which later will used as a substrate in fermentation process. Fermentation of lactic acid was carried out using Lactobacillus rhamnosus as the lactic acid microbes and this process was designed using Central Composite Design CCD from Design Expert v6.0.8 software. The highest yield oflactic acid which is 7.02 giL was occurred at run 5 when the value of peptone and yeast extract is at highest.

Additional Metadata

[error in script]
Item Type: Proceedings Paper
Additional Information: Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. mal TP339 B615 Call Number
AGROVOC Term: Lactic acid
AGROVOC Term: Kenaf
AGROVOC Term: Lactobacillus
AGROVOC Term: Carboxylic acids
AGROVOC Term: Biodegradability
AGROVOC Term: Biomass
AGROVOC Term: Fermentation
AGROVOC Term: Yeasts
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:14
URI: http://webagris.upm.edu.my/id/eprint/12124

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