The application of molecular techniques in the diagnosis of black pepper Piper nigrum L. viruses in Sarawak

Jadol John, . and Eng Lily, . and Ong Ching-Ang, . and Jones Phil, . (1999) The application of molecular techniques in the diagnosis of black pepper Piper nigrum L. viruses in Sarawak. [Proceedings Paper]

Black pepper Piper nigrum L. in Sarawak can be infected by 2 types of viruses a cucumovirus and a badnavirus. The absence of antibodies for testing coupled with the high phenolic and polysaccharide contents in the plant make diagnosis of these viruses difficult. The successful application of dsRNA and PCR techniques to detect the cucumovirus and the badnavirus respectively has enabled diagnosis and the screening of planting material to be carried out by the Department of Agriculture in Sarawak.

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Abstract

Black pepper Piper nigrum L. in Sarawak can be infected by 2 types of viruses a cucumovirus and a badnavirus. The absence of antibodies for testing coupled with the high phenolic and polysaccharide contents in the plant make diagnosis of these viruses difficult. The successful application of dsRNA and PCR techniques to detect the cucumovirus and the badnavirus respectively has enabled diagnosis and the screening of planting material to be carried out by the Department of Agriculture in Sarawak.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. Call number mal SB 950 I61 1999
AGROVOC Term:	Black pepper
AGROVOC Term:	Piper nigrum
AGROVOC Term:	Screening tests
AGROVOC Term:	Antibodies
AGROVOC Term:	Phenolic content
AGROVOC Term:	Lipopolysaccharides
AGROVOC Term:	Cucumoviruses
AGROVOC Term:	Planting
AGROVOC Term:	Diagnosis
AGROVOC Term:	Phenols
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:14
URI:	http://webagris.upm.edu.my/id/eprint/12220

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