Citation
Thomas S., . and Balasundaran M., . (1999) Immunological detection of sandal spike phytoplasma. [Proceedings Paper]
Abstract
Spike disease caused by a non-culturable phytoplasma is the most serious disease of sandal Santalum album L. known for its scented heartwood. An immunological method was developed for early detection of the pathogen in infected plants. The phytoplasma was purified by a differential filtration technique and ultra-centrifugation. The purity of the phytoplasma pellet was confirmed using scanning and transmission electron microscopy. Polyclonal antibodies raised in rabbits against purified phytoplasma were used to detect the pathogen present in sandal using direct ELISA technique. The effect of duration on antigen coating and incubation with conjugated antibody in ELISA plates showed that three hours duration was optimum.
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Abstract
Spike disease caused by a non-culturable phytoplasma is the most serious disease of sandal Santalum album L. known for its scented heartwood. An immunological method was developed for early detection of the pathogen in infected plants. The phytoplasma was purified by a differential filtration technique and ultra-centrifugation. The purity of the phytoplasma pellet was confirmed using scanning and transmission electron microscopy. Polyclonal antibodies raised in rabbits against purified phytoplasma were used to detect the pathogen present in sandal using direct ELISA technique. The effect of duration on antigen coating and incubation with conjugated antibody in ELISA plates showed that three hours duration was optimum.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. Call number mal SB 950 I61 1999 |
| AGROVOC Term: | Immunology |
| AGROVOC Term: | Phytoplasmas |
| AGROVOC Term: | Santalum album |
| AGROVOC Term: | Heartwood |
| AGROVOC Term: | Filtration |
| AGROVOC Term: | Centrifugation |
| AGROVOC Term: | Pellets |
| AGROVOC Term: | Scanning microscopy |
| AGROVOC Term: | Electron microscopy |
| AGROVOC Term: | Polyclonal antibodies |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:14 |
| URI: | http://webagris.upm.edu.my/id/eprint/12260 |
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