Citation
Norani Abdul-Samad, . and Tan Lee Hoi, . (1999) Evaluation of filter paper sero-assay FIPSA to detect three viruses. [Proceedings Paper]
Abstract
The filter paper sero-assay FiPSA was examined tested and compared with three different plant viruses; Clitoria yellow vein virus CYW Hibiscus chlorotic ringspot virus HCRY and tomato mosaic virus ToMY in purified and crude sap preparations. The filter paper strips that were preblocked were found to increase the sensitivity of FiPSA as the background were suppressed or reduced when compared to strips without pre-blocking in blocking solutions. Positive test was observed as a violet-blue spot. By using pre-blocked paper strips the purified viruses and sap extracts of CYVV HCRV and ToMV could be detected up to 3.0 ng/ml and 1: 10 000 dilution 15.0 ng/ml and 1:4 800 dilution 5.0 ng/rnl and 1:8 000 dilution respectively.
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Abstract
The filter paper sero-assay FiPSA was examined tested and compared with three different plant viruses; Clitoria yellow vein virus CYW Hibiscus chlorotic ringspot virus HCRY and tomato mosaic virus ToMY in purified and crude sap preparations. The filter paper strips that were preblocked were found to increase the sensitivity of FiPSA as the background were suppressed or reduced when compared to strips without pre-blocking in blocking solutions. Positive test was observed as a violet-blue spot. By using pre-blocked paper strips the purified viruses and sap extracts of CYVV HCRV and ToMV could be detected up to 3.0 ng/ml and 1: 10 000 dilution 15.0 ng/ml and 1:4 800 dilution 5.0 ng/rnl and 1:8 000 dilution respectively.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. Call number mal SB 950 I61 1999 |
| AGROVOC Term: | Clitoria |
| AGROVOC Term: | Hibiscus |
| AGROVOC Term: | Tomato mosaic virus |
| AGROVOC Term: | Serological techniques |
| AGROVOC Term: | Plant viruses |
| AGROVOC Term: | Infection |
| AGROVOC Term: | Viruses |
| AGROVOC Term: | Antigens |
| AGROVOC Term: | Immunoassay |
| AGROVOC Term: | Densitometry |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12322 |
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