Studies on cellular behaviour in regenerative and non-regenerative tissues of Dianthus caryophyllus linn. cv. Grenadin in tissue culture system


Citation

Rosna M. T., . and Azrnan L., . and Norfariza A. K., . (2001) Studies on cellular behaviour in regenerative and non-regenerative tissues of Dianthus caryophyllus linn. cv. Grenadin in tissue culture system. [Proceedings Paper]

Abstract

Regenerative tissues are those that are capable to regenerate into complete plants while those that fail to do likewise are considered as non-regenerative tissues. In this paper some cellular activities such as Mitotic Index chromosome counts cell doubling times nuclear DNA content and mean cell and nuclear areas were examined to compare these two types of tissues. Our results showed that the cell doubling time was faster and the nuclear to cell area ratios were constant in regenerative tissues whereas other parameters were almost similar in both tissue types. Dianthus caryophyllus carnation was found to be stable when their tissues are brought into culture system indicating that clonal propagation is highly achievable. Although the occurrence of polyploidy was very high in regenerative tissues after 3 weeks 2 months and 3 months in culture complete plant regeneration could still be accomplished.


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Abstract

Regenerative tissues are those that are capable to regenerate into complete plants while those that fail to do likewise are considered as non-regenerative tissues. In this paper some cellular activities such as Mitotic Index chromosome counts cell doubling times nuclear DNA content and mean cell and nuclear areas were examined to compare these two types of tissues. Our results showed that the cell doubling time was faster and the nuclear to cell area ratios were constant in regenerative tissues whereas other parameters were almost similar in both tissue types. Dianthus caryophyllus carnation was found to be stable when their tissues are brought into culture system indicating that clonal propagation is highly achievable. Although the occurrence of polyploidy was very high in regenerative tissues after 3 weeks 2 months and 3 months in culture complete plant regeneration could still be accomplished.

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK 710.2 M239 2001 Call Number
AGROVOC Term: Dianthus caryophyllus
AGROVOC Term: Carnations
AGROVOC Term: Explants
AGROVOC Term: In vitro culture
AGROVOC Term: In vitro regeneration
AGROVOC Term: Experiments
AGROVOC Term: Leaves
AGROVOC Term: Micropropagation
AGROVOC Term: Plant growth substances
AGROVOC Term: Roots
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:15
URI: http://webagris.upm.edu.my/id/eprint/12490

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