Citation
Shahsavari E., . and Mirlohi A. F., . and Khayyam Nekoei M., . (2008) Cross inoculation of two tall fescue genotypes with Neotyphodium endophytic fungi and its detection using AFLPS marker. [Proceedings Paper]
Abstract
Mutual relationship between pasture grasses and fungal endophytes of genus Neotyphodium are one of the most important symbiosis in nature. Endophytes have many advantageous effects such as pest and disease resistance and resistance to environmental stresses for their hosts. The purpose of this research was to evaluate and compare capabilities of different cross inoculation methods. Two Iranian genotypes of tall fescue known as 75 and 83 were selected and their endophytes were isolated. Three methods of cross inoculation were used in this research consisting of Latch and Christensen approach insertion of mycelium into the seeds and Johanson -Cicales et al approach. Endophytes were detected in new host using microscopic examination of stained tissues AFLP marker and Kokh;s postulates. Results showed that Latch and Christensen approach was the only technique by which endophytes were successfully inoculated into both original and new host genotypes. Detection methods used to confirm the presence of endophytic fungi in plants indicated that microscopic examination was the most efficient. AFLPs marker detected presence of endophyte in some plants but not in all. Kokh;s postulates showed that endophytic fungi had no morphological alterations after being inoculated into new host.
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Abstract
Mutual relationship between pasture grasses and fungal endophytes of genus Neotyphodium are one of the most important symbiosis in nature. Endophytes have many advantageous effects such as pest and disease resistance and resistance to environmental stresses for their hosts. The purpose of this research was to evaluate and compare capabilities of different cross inoculation methods. Two Iranian genotypes of tall fescue known as 75 and 83 were selected and their endophytes were isolated. Three methods of cross inoculation were used in this research consisting of Latch and Christensen approach insertion of mycelium into the seeds and Johanson -Cicales et al approach. Endophytes were detected in new host using microscopic examination of stained tissues AFLP marker and Kokh;s postulates. Results showed that Latch and Christensen approach was the only technique by which endophytes were successfully inoculated into both original and new host genotypes. Detection methods used to confirm the presence of endophytic fungi in plants indicated that microscopic examination was the most efficient. AFLPs marker detected presence of endophyte in some plants but not in all. Kokh;s postulates showed that endophytic fungi had no morphological alterations after being inoculated into new host.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2008 Call Number |
| AGROVOC Term: | Inoculation |
| AGROVOC Term: | Genotypes |
| AGROVOC Term: | Fescues |
| AGROVOC Term: | Fungi |
| AGROVOC Term: | Grasses |
| AGROVOC Term: | Endophytes |
| AGROVOC Term: | Symbiosis |
| AGROVOC Term: | Mycelium |
| AGROVOC Term: | Seeds |
| AGROVOC Term: | Hosts |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12766 |
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