Isolation of papaya carboxy-terminal protease CDNA clone during ripening

Roohaida O., . and Chong H. L., . (2004) Isolation of papaya carboxy-terminal protease CDNA clone during ripening. [Proceedings Paper]

A novel type of serine protease with a Ser/Lys catalytic dyad is responsible for the C-terminal processing of precursor Dl protein pDl of the photosystem II reaction center a process that is indispensable for the integration of water-splitting machinery in photosynthesis. A papaya cDNA clone coding for a carboxy terminal protease has been isolated from a ripening papaya cDNA library. The expected amino acid sequence shared homology with carboxy-terminal protease gene from Arabidopsis thaliana Spinacia oleracea and Scenedesmus obliquus which may be involved in removal of carboxy-terminal extension of the D1 protein in photosystem II PSII. Subsequent sequence comparisons with other plant cDNA sequences showed that the papaya cDNA clone aligned to the middle part of other plant proteases hence indicating truncation at the 5;-end of the clone. Further analysis of the papaya protease sequence showed presence of the catalytic dyad Ser/Lys a feature common to this enzyme family.

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Abstract

A novel type of serine protease with a Ser/Lys catalytic dyad is responsible for the C-terminal processing of precursor Dl protein pDl of the photosystem II reaction center a process that is indispensable for the integration of water-splitting machinery in photosynthesis. A papaya cDNA clone coding for a carboxy terminal protease has been isolated from a ripening papaya cDNA library. The expected amino acid sequence shared homology with carboxy-terminal protease gene from Arabidopsis thaliana Spinacia oleracea and Scenedesmus obliquus which may be involved in removal of carboxy-terminal extension of the D1 protein in photosystem II PSII. Subsequent sequence comparisons with other plant cDNA sequences showed that the papaya cDNA clone aligned to the middle part of other plant proteases hence indicating truncation at the 5;-end of the clone. Further analysis of the papaya protease sequence showed presence of the catalytic dyad Ser/Lys a feature common to this enzyme family.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2004 Call Number
AGROVOC Term:	Carica papaya
AGROVOC Term:	Papayas
AGROVOC Term:	Ripening
AGROVOC Term:	Isolation
AGROVOC Term:	Proteases
AGROVOC Term:	Extraction
AGROVOC Term:	DNA cloning
AGROVOC Term:	Amino acids
AGROVOC Term:	Catalytic activity
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/12820

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