Citation
Rosilah A. A., . and Kandasamy K. I., . (2004) Mass propagation of Sungkai Peronema canescens for plantation forestry using tissue culture methods. [Proceedings Paper]
Abstract
Sungkai Peronema canescens is an indigenous fast-growing semi hard-wood species. It is an attractive species for establishing forest plantation models especially due to its fast growing nature and desirable wood quality e.g. its whitecoloured wood popular amongst chop-stick makers. Since the availability of highquality planting materials are a pre-requisite for any forest plantation programmes thus it was necessary to develop an efficient mass propagation method for this species. Cuttings of selected trees from Sabah were established in FRIM;s nursery and the resulting plants were used as the explant source for micropropagation experiments. Clean cultures were established using suiface sterilised nodal cuttings. Resulting clean shoot cultures were used for the development of a suitable shoot-multiplication media formulation including different basal medium types and various concentration and combinations of growth regulators. The incorporation of 0.5mg/L BAP in MS medium was found to be most effective for the induction of shoot multiplication. A sub-culturing cycle of six 6 weeks were adopted for the purpose of mass propagation of these species. MS medium supplemented with 0.1 mg/L BAP was sufficient to induce shoot elongation thus producing well-formed shoot cultures which could then be used either for further multiplication or rooting activities. Shoot cultures rooted well within four 4 weeks upon being cultured on MS medium supplemented with 1mg/L IBA. Complete plantlets were successfully weaned off in our own Award-wining Weaning Chamber in our greenhouses. With this system were have been able to produce over 10 000 plants for our customers within the last six 6 months.
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Abstract
Sungkai Peronema canescens is an indigenous fast-growing semi hard-wood species. It is an attractive species for establishing forest plantation models especially due to its fast growing nature and desirable wood quality e.g. its whitecoloured wood popular amongst chop-stick makers. Since the availability of highquality planting materials are a pre-requisite for any forest plantation programmes thus it was necessary to develop an efficient mass propagation method for this species. Cuttings of selected trees from Sabah were established in FRIM;s nursery and the resulting plants were used as the explant source for micropropagation experiments. Clean cultures were established using suiface sterilised nodal cuttings. Resulting clean shoot cultures were used for the development of a suitable shoot-multiplication media formulation including different basal medium types and various concentration and combinations of growth regulators. The incorporation of 0.5mg/L BAP in MS medium was found to be most effective for the induction of shoot multiplication. A sub-culturing cycle of six 6 weeks were adopted for the purpose of mass propagation of these species. MS medium supplemented with 0.1 mg/L BAP was sufficient to induce shoot elongation thus producing well-formed shoot cultures which could then be used either for further multiplication or rooting activities. Shoot cultures rooted well within four 4 weeks upon being cultured on MS medium supplemented with 1mg/L IBA. Complete plantlets were successfully weaned off in our own Award-wining Weaning Chamber in our greenhouses. With this system were have been able to produce over 10 000 plants for our customers within the last six 6 months.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2004 Call Number |
| AGROVOC Term: | Lamiaceae |
| AGROVOC Term: | Hardwood |
| AGROVOC Term: | Plant propagation |
| AGROVOC Term: | Tissue culture |
| AGROVOC Term: | Plant multiplication |
| AGROVOC Term: | Shoot tip culture |
| AGROVOC Term: | Rooting |
| AGROVOC Term: | Explants |
| AGROVOC Term: | Forest plantations |
| AGROVOC Term: | Wood products |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12842 |
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