Citation
Ismanizan Ismail, . and Zamri Zainal, . and Roohaida Othman, . and Roslinda Sajari, . and Nik Marzuki Sidik, . (2000) Isolation of a set of putative ripening-related e-DNA clones from chilli by differential screening. [Proceedings Paper]
Abstract
The ripening of chilli Capsicum annuum L. is brought about by many changes in gene expression. To understand how these changes affect the biochemistry and physiology of the fruit the specific genes involved have to be examined. In this study a cDNA library prepared from chilli fruit was differentially screened for ripening related clones using cDNA from unripe and ripe fruits. This has led to the isolation of 47 clones obtained in the primary screening. Following that 10 differentially expressed clones were isolated from the secondary screening. The partial sequences of four of these cDNAs were compared with database sequences and they showed homology to homeobox leucine zipper 0.6kb ornithine decarboxylase 1.6kb elongation factor EF-l 1.4kb and glucose 6-phosphate dehydrogenase 1.8kb. These cDNA clones were designated as cUKMI0 cUKMll cUKMI2 and cUKM13 respectively.
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Abstract
The ripening of chilli Capsicum annuum L. is brought about by many changes in gene expression. To understand how these changes affect the biochemistry and physiology of the fruit the specific genes involved have to be examined. In this study a cDNA library prepared from chilli fruit was differentially screened for ripening related clones using cDNA from unripe and ripe fruits. This has led to the isolation of 47 clones obtained in the primary screening. Following that 10 differentially expressed clones were isolated from the secondary screening. The partial sequences of four of these cDNAs were compared with database sequences and they showed homology to homeobox leucine zipper 0.6kb ornithine decarboxylase 1.6kb elongation factor EF-l 1.4kb and glucose 6-phosphate dehydrogenase 1.8kb. These cDNA clones were designated as cUKMI0 cUKMll cUKMI2 and cUKM13 respectively.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2000 Call Number |
| AGROVOC Term: | Chillies |
| AGROVOC Term: | Capsicum annuum |
| AGROVOC Term: | Ripening |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | DNA |
| AGROVOC Term: | Clones |
| AGROVOC Term: | Genes |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12898 |
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