Citation
Nor Salina M. Z., . and Daud S. K., . and Marziah M., . (2000) Allozymic variation in banana cultivars. [Proceedings Paper]
Abstract
Allozyme variation among and within 3 varieties of wild banana namely berangan AA group nangka AAB group and novaria AAA group were detected using horizontal starch gel electrophoresis. Genetic variation between somaclonal and wild bananas of the same varieties were also compared to determine any changes in their genetic composition. Of 14 loci examined 6 loci 43 were found to be polymorphic P 095 at least in one of the varieties. They are GPl MDH-l . MDH-2. EST-l . EST-2 and ALP-l . Of seven enzyme systems only 4 exhibited scorable loci. The mean heterozygosity per locus were 0.287 0.417 and 0.235 for berangan nangka and novaria respectively. In berangan and novaria the number of loci were generally reduced in their somaclonal samples compared to the wild ones especially loci produced by MDH EST and ALP enzymes. In nangka the number of loci produced by GPI and EST enzymes was greater in the somaclonal samples compared to the wild samples. Dendrogram based on genetic distances and UPGMA showed that berangan and novaria were clustered together with the genetic distance of 0.099 while nangka was distantly separated from the other two varieties with the genetic distance of 0.320.
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Abstract
Allozyme variation among and within 3 varieties of wild banana namely berangan AA group nangka AAB group and novaria AAA group were detected using horizontal starch gel electrophoresis. Genetic variation between somaclonal and wild bananas of the same varieties were also compared to determine any changes in their genetic composition. Of 14 loci examined 6 loci 43 were found to be polymorphic P 095 at least in one of the varieties. They are GPl MDH-l . MDH-2. EST-l . EST-2 and ALP-l . Of seven enzyme systems only 4 exhibited scorable loci. The mean heterozygosity per locus were 0.287 0.417 and 0.235 for berangan nangka and novaria respectively. In berangan and novaria the number of loci were generally reduced in their somaclonal samples compared to the wild ones especially loci produced by MDH EST and ALP enzymes. In nangka the number of loci produced by GPI and EST enzymes was greater in the somaclonal samples compared to the wild samples. Dendrogram based on genetic distances and UPGMA showed that berangan and novaria were clustered together with the genetic distance of 0.099 while nangka was distantly separated from the other two varieties with the genetic distance of 0.320.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK710.2 M239 2000 Call Number |
| AGROVOC Term: | Bananas |
| AGROVOC Term: | Musaceae |
| AGROVOC Term: | Musa bananas |
| AGROVOC Term: | Genetic variation |
| AGROVOC Term: | Allozymes |
| AGROVOC Term: | Gel electrophoresis |
| AGROVOC Term: | Genetic differences |
| AGROVOC Term: | Polymorphism |
| AGROVOC Term: | Somaclonal variation |
| AGROVOC Term: | Loci |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/12943 |
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