In vitro screening of antagonistic yeast for biological control of anthracnose caused by Colletotrichum gloeosporioides in ;Frangi; papaya


Citation

Hamizah Hassan, . and Mahmud Tengku Muda Mohamed, . and Kamaruzaman Sijam, . and Siti Hajar Ahmad, . (2011) In vitro screening of antagonistic yeast for biological control of anthracnose caused by Colletotrichum gloeosporioides in ;Frangi; papaya. [Proceedings Paper]

Abstract

Postharvest fruit decay causes decrease in both quantity and quality of the fruits. Thus it is a major constraint in papaya production. Based on previous study anthracnose caused by Colletotrichum gloeosporioides was the most prevalent postharvest fungal disease attacking ;Frangi; papaya where disease incidence and severity was recorded as 90-98 and 25-38 respectively and the magnitude increased as the fruits ripened. Conventional fungicide treatment is losing popularity due to high residual problem and detrimental to the environment. Thus a study was conducted to look for suitable strain of yeast that can substitute fungicides in controlling the disease. The objectives of this study were to isolate select and test the in-vitro ability of epiphytic microorganisms isolated from papaya fruit leaf stem and trunk in controlling anthracnose onset after harvest. A total of 110 strains of yeasts were obtained and 29 showed antagonistic activity against C. gloeosporioides in-vitro. Out of the 29 yeasts only five strains YK YC YT YA and YW showed high percent inhibition of radial growth 70.3 0.608 0.595 59.5 and 59.5 respectively in dual culture assay and further assayed by well test. From the well test YK gave the strongest antifungal effect which no mycelial growth observed and YW gave the lowest with 4.73 cm mycelial growth.


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Abstract

Postharvest fruit decay causes decrease in both quantity and quality of the fruits. Thus it is a major constraint in papaya production. Based on previous study anthracnose caused by Colletotrichum gloeosporioides was the most prevalent postharvest fungal disease attacking ;Frangi; papaya where disease incidence and severity was recorded as 90-98 and 25-38 respectively and the magnitude increased as the fruits ripened. Conventional fungicide treatment is losing popularity due to high residual problem and detrimental to the environment. Thus a study was conducted to look for suitable strain of yeast that can substitute fungicides in controlling the disease. The objectives of this study were to isolate select and test the in-vitro ability of epiphytic microorganisms isolated from papaya fruit leaf stem and trunk in controlling anthracnose onset after harvest. A total of 110 strains of yeasts were obtained and 29 showed antagonistic activity against C. gloeosporioides in-vitro. Out of the 29 yeasts only five strains YK YC YT YA and YW showed high percent inhibition of radial growth 70.3 0.608 0.595 59.5 and 59.5 respectively in dual culture assay and further assayed by well test. From the well test YK gave the strongest antifungal effect which no mycelial growth observed and YW gave the lowest with 4.73 cm mycelial growth.

Additional Metadata

[error in script]
Item Type: Proceedings Paper
Additional Information: Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term: Carica
AGROVOC Term: Carica papaya
AGROVOC Term: Papayas
AGROVOC Term: Colletotrichum gloeosporioides
AGROVOC Term: In vitro
AGROVOC Term: Biological control
AGROVOC Term: Biological control agents
AGROVOC Term: Disease incidence
AGROVOC Term: Fungi
AGROVOC Term: Fungal diseases
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:15
URI: http://webagris.upm.edu.my/id/eprint/12999

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