Isolation and identification of paraquat degrading microorganisms from contaminated soil

Nur Natisha Zolkefli, . and Darah Ibrahim, . (2011) Isolation and identification of paraquat degrading microorganisms from contaminated soil. [Proceedings Paper]

Paraquat is a quaternary nitrogen compound known by its chemical name 1 1;dimethyl-4 4;-bipyridylium dichloride a controversial herbicide used globally in most countries without restrictions. The xenobiotic paraquat used extensively on a wide variety of agricultural crops as a non-selective broad spectrum herbicide adversely affect cell membranes via lipid peroxidation and affects also protein and DNA. It is believed generally that the herbicide paraquat is quickly inactivated after being incorporated in soil and becomes unavailable to microorganisms since it is tightly adsorbed to soil. However the paraquat in soil solution is intrinsically biodegradable being rapidly and completely mineralised by soil microorganisms. The output of the study was to isolate and identify the indigenous active paraquat degrading bacteria from paraquat contaminated soil. From the preliminary screening 44 potential paraquat degraders were isolated. During the secondary screening 3 most potential paraquat degraders were chosen to be identified. Phylogenetic analysis of 168 rRNA using both 5; forward and 3; reverse sequences showed that these bacteria were closely related to Bacillus cereus and Sinomonas atrocyanea respectively. S. atrocyanea was selected as the best indigenous paraquat degrader. Thus S. atrocyanea was used to determine the degradation activity via solid-phase extraction and the remaining paraquat extracted can be detected spectrometrically at A‚‚‚.

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Abstract

Paraquat is a quaternary nitrogen compound known by its chemical name 1 1;dimethyl-4 4;-bipyridylium dichloride a controversial herbicide used globally in most countries without restrictions. The xenobiotic paraquat used extensively on a wide variety of agricultural crops as a non-selective broad spectrum herbicide adversely affect cell membranes via lipid peroxidation and affects also protein and DNA. It is believed generally that the herbicide paraquat is quickly inactivated after being incorporated in soil and becomes unavailable to microorganisms since it is tightly adsorbed to soil. However the paraquat in soil solution is intrinsically biodegradable being rapidly and completely mineralised by soil microorganisms. The output of the study was to isolate and identify the indigenous active paraquat degrading bacteria from paraquat contaminated soil. From the preliminary screening 44 potential paraquat degraders were isolated. During the secondary screening 3 most potential paraquat degraders were chosen to be identified. Phylogenetic analysis of 168 rRNA using both 5; forward and 3; reverse sequences showed that these bacteria were closely related to Bacillus cereus and Sinomonas atrocyanea respectively. S. atrocyanea was selected as the best indigenous paraquat degrader. Thus S. atrocyanea was used to determine the degradation activity via solid-phase extraction and the remaining paraquat extracted can be detected spectrometrically at A‚‚‚.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term:	Paraquat
AGROVOC Term:	Degradation
AGROVOC Term:	Microorganisms
AGROVOC Term:	Contaminated soils
AGROVOC Term:	Herbicides
AGROVOC Term:	Isolation
AGROVOC Term:	Identification
AGROVOC Term:	Inoculation
AGROVOC Term:	Soil sampling
AGROVOC Term:	Screening tests
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13000

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