Citation
Nakisah Mat Amin, . and Nur Nadia Abu Bakar, . and Cha Thye San, . (2011) Assessment of toxicity of zinc and lead on Acanthamoeba polyphaga. [Proceedings Paper]
Abstract
Heavy metals such as lead and zinc are abundant in the aquatic environment and affect the life of the organisms including free-living amoebae both at cellular and genomic level thus lead to a decline of their population size. This study examined the effect of zinc and lead on the DNA of Acanthamoeba polyphaga CCAP 1501/3A using comet and DNA laddering assays. The amoeba was exposed to a series of concentrations of zinc and lead in 24-well plates for 72 h before the amoeba viability and the IC‚‚ values of the metals against the amoeba were determined. The viability of amoebae was decreased in response to the increasing of heavy metals concentrations. The IC‚o value of zinc against this amoeba was 52.0 ppm and for lead was 15.0 ppm. Assessment done by comet and DNA laddering assays demonstrated that both heavy metals affect the amoeba both at its cellular and genomic level and induce apoptosis type of cell death.
Download File
Full text available from:
|
Abstract
Heavy metals such as lead and zinc are abundant in the aquatic environment and affect the life of the organisms including free-living amoebae both at cellular and genomic level thus lead to a decline of their population size. This study examined the effect of zinc and lead on the DNA of Acanthamoeba polyphaga CCAP 1501/3A using comet and DNA laddering assays. The amoeba was exposed to a series of concentrations of zinc and lead in 24-well plates for 72 h before the amoeba viability and the IC‚‚ values of the metals against the amoeba were determined. The viability of amoebae was decreased in response to the increasing of heavy metals concentrations. The IC‚o value of zinc against this amoeba was 52.0 ppm and for lead was 15.0 ppm. Assessment done by comet and DNA laddering assays demonstrated that both heavy metals affect the amoeba both at its cellular and genomic level and induce apoptosis type of cell death.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Acanthamoeba |
| AGROVOC Term: | Protozoa |
| AGROVOC Term: | Amoeba |
| AGROVOC Term: | Heavy metals |
| AGROVOC Term: | Zinc |
| AGROVOC Term: | Lead |
| AGROVOC Term: | Cultivation |
| AGROVOC Term: | Cytotoxicity |
| AGROVOC Term: | DNA |
| AGROVOC Term: | Evaluation |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13001 |
Actions (login required)
 |
View Item |
