Citation
Ahmad Lutfi Lukman, . and Abu Bakar Salleh, . and Raja Noor Zaliha Raja Abd. Rahman, . and Mahiran Basri, . (2011) Biochemical screening for metalworking fluid degraders. [Proceedings Paper]
Abstract
Metalworking fluid MWF is a major scheduled waste in metalworking industry with more than 20 billion L being produced worldwide annually. The disposal cost of the waste is high due to its oil content. Biodegradation is difficult due to its toxicity and the presence of biocides. Thus the aim of this study is to perform bioaugmentation using hydrocarbon-utilising bacteria isolated from oil-contaminated environment. Soil and 01 samples were collected from a workshop and boat jetty in Terengganu. Preliminary screening was done via enrichment culture with 10 MWF waste. A total of 17 and 11 bacteria were isolated from the workshop and jetty respectively. Biodegradative ability of these bacteria were qualitatively tested on three types of MWF waste at 10 50c: and 100 concentrations with 0.05 2 3 5-triphenyl tetrazolium chloride as growth indicator. From a total of 28 isolates tested 10 bacteria were found to have good growth in 10 MWFs waste exhibited by the red colour of formazan a water-insoluble azo compound formed by reduction of tetrazolium salt. However increasing the concentrations of MWFs waste to 100 was proven to inhibit bacterial growth. Only 8 7 and 2 strains were able to withstand 100 of MWF1 MWF2 and MWF3 respectively.
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Abstract
Metalworking fluid MWF is a major scheduled waste in metalworking industry with more than 20 billion L being produced worldwide annually. The disposal cost of the waste is high due to its oil content. Biodegradation is difficult due to its toxicity and the presence of biocides. Thus the aim of this study is to perform bioaugmentation using hydrocarbon-utilising bacteria isolated from oil-contaminated environment. Soil and 01 samples were collected from a workshop and boat jetty in Terengganu. Preliminary screening was done via enrichment culture with 10 MWF waste. A total of 17 and 11 bacteria were isolated from the workshop and jetty respectively. Biodegradative ability of these bacteria were qualitatively tested on three types of MWF waste at 10 50c: and 100 concentrations with 0.05 2 3 5-triphenyl tetrazolium chloride as growth indicator. From a total of 28 isolates tested 10 bacteria were found to have good growth in 10 MWFs waste exhibited by the red colour of formazan a water-insoluble azo compound formed by reduction of tetrazolium salt. However increasing the concentrations of MWFs waste to 100 was proven to inhibit bacterial growth. Only 8 7 and 2 strains were able to withstand 100 of MWF1 MWF2 and MWF3 respectively.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Metals |
| AGROVOC Term: | Fluids |
| AGROVOC Term: | Biochemistry |
| AGROVOC Term: | Industrial wastes |
| AGROVOC Term: | Hydrocarbons |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | Biodegradation |
| AGROVOC Term: | Stones |
| AGROVOC Term: | Oils |
| AGROVOC Term: | Sampling |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13014 |
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