Citation
Saw Suet Yit, . and Lee Choon Weng, . (2011) Archaea diversity in tropical coastal waters. [Proceedings Paper]
Abstract
The diversity of archaeal communities at Port Klang Port Dickson Sungai Muar and Kuala Selangor was investigated with the construction of 16S rDNA gene clones libraries with archaea-specific primers. In total 1139 randomly selected clones were screened by PCR-restriction fragment length polymorphism PCR-RFLP analysis using three different enzymes Rsal Cfol and Ddel. By the values of Shannon diversity H index it was demonstrated that Sungai Muar H 3.54 and Kuala Selangor H 3.49 clone library had relatively higher diversity of the archaeal operational taxonomic units OTUs compared to Port Dickson H 2.55 and Port Klang H 2.48. Selected phylotypes in this study shared high similarity 97 to Nitrosotalea devanaterra Nitrosopumilus sp. Methanosaeta concilii and uncultured archaeon clone sequences. We are currently carrying out further investigations on the effects of different physico-chemical characteristics on the spatial distribution of archaea.
Download File
Full text available from:
|
Abstract
The diversity of archaeal communities at Port Klang Port Dickson Sungai Muar and Kuala Selangor was investigated with the construction of 16S rDNA gene clones libraries with archaea-specific primers. In total 1139 randomly selected clones were screened by PCR-restriction fragment length polymorphism PCR-RFLP analysis using three different enzymes Rsal Cfol and Ddel. By the values of Shannon diversity H index it was demonstrated that Sungai Muar H 3.54 and Kuala Selangor H 3.49 clone library had relatively higher diversity of the archaeal operational taxonomic units OTUs compared to Port Dickson H 2.55 and Port Klang H 2.48. Selected phylotypes in this study shared high similarity 97 to Nitrosotalea devanaterra Nitrosopumilus sp. Methanosaeta concilii and uncultured archaeon clone sequences. We are currently carrying out further investigations on the effects of different physico-chemical characteristics on the spatial distribution of archaea.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Microorganisms |
| AGROVOC Term: | Clones |
| AGROVOC Term: | DNA cloning |
| AGROVOC Term: | Enzymes |
| AGROVOC Term: | Coastal area |
| AGROVOC Term: | Coastal waters |
| AGROVOC Term: | Microbial flora |
| AGROVOC Term: | Sampling |
| AGROVOC Term: | Dissolved oxygen |
| AGROVOC Term: | Spectrophotometers |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13015 |
Actions (login required)
 |
View Item |
