Retrobiosynthetic analysis approach for metabolic pathway construction of Lactococcus lactis grown in various conditions

Kamalrul Azlan Azizan, . and Syarul Nataqain Baharum, . and Normah Mohd Nor, . (2011) Retrobiosynthetic analysis approach for metabolic pathway construction of Lactococcus lactis grown in various conditions. [Proceedings Paper]

Focusing on small molecular weight metabolic products a retrobiosynthetic analysis approach was applied to metabolomics data of Lactococcus lactis to construct a metabolic pathway. The data generated from comprehensive chromatography techniques coupled to mass spectrometry instrument were firstly validated discriminated and correlatively established using multivariate analysis of Principal Component Analysis PCA and Partial Least Squares Discriminant Analysis PLSDA. The results of the analyses suggested that metabolites with lower loading column values gave higher metabolic changes thus affecting the way multivariate modeling was separated and clustered. It was found that the heterofermentation products were the major contributor governing the extracellular followed by products derived from butanoate and propanoate metabolism. Furthermore application of two data scaling methods for further discrimination and complementary information was discussed in detail.

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Abstract

Focusing on small molecular weight metabolic products a retrobiosynthetic analysis approach was applied to metabolomics data of Lactococcus lactis to construct a metabolic pathway. The data generated from comprehensive chromatography techniques coupled to mass spectrometry instrument were firstly validated discriminated and correlatively established using multivariate analysis of Principal Component Analysis PCA and Partial Least Squares Discriminant Analysis PLSDA. The results of the analyses suggested that metabolites with lower loading column values gave higher metabolic changes thus affecting the way multivariate modeling was separated and clustered. It was found that the heterofermentation products were the major contributor governing the extracellular followed by products derived from butanoate and propanoate metabolism. Furthermore application of two data scaling methods for further discrimination and complementary information was discussed in detail.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number
AGROVOC Term:	Lactococcus
AGROVOC Term:	Lactococcus lactis
AGROVOC Term:	Streptococcaceae
AGROVOC Term:	Gram positive bacteria
AGROVOC Term:	Fermentation
AGROVOC Term:	analysis
AGROVOC Term:	Data processing
AGROVOC Term:	Chromatography
AGROVOC Term:	Mass spectrometry
AGROVOC Term:	Analytical methods
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13030

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