Molecular characterisation of Vibrio parahaemolyticus carrying TDH and TRH gene

Keng Teck Ee, . and Radu Son, . and Nishibuchi Mitsuaki, . and Cheah Yoke Kqueen, . (2011) Molecular characterisation of Vibrio parahaemolyticus carrying TDH and TRH gene. [Proceedings Paper]

Vibrio parahaemolyticus is a marine bacterium heavily documented for the presence of Thermostable Direct Haemolysin Gene tdh and Thermostable Direct Haemolysinrelated Haemolysin Gene trh since the 1970s. It is epidemiologically worldwide that spreads through poor sanitation and unhygienic marketplaces in Japan India United States Mexico Indonesia and even Malaysia. This bacterium is often associated with infection via faecal-oral route that causes an array of gastroenteritis symptoms which includes diarrhoea vomiting fever and abdominal pain. Although this bacterium is heat sensitive and can be prevented through hygienic practices it poses a potential public health threat in rural communities and in times of flood disasters. Understanding this bacterium better would enable more efficient emergency care during outbreaks. The purpose of this study is to characterise V. parahaemolyticus carrying tdh and trh gene using molecular typing methods thus determine their clonal relatedness. It is hypothesised that there is specific clonal relatedness among V. parahaemolyticus carrying tdh and trh gene with respect to Random Amplification of Polymorphic DNA RAPD and Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction ERIC-PCR fingerprinting. Using V. parahaemolyticus isolates carrying tdh and trh gene which are collected from previous studies on bloody clam Anadara granosaand Lala Orbicularia orbiculata 33 potential isolates are revived using Tryptic Soy Broth with 3 sodium chloride. DNA is extracted using simple boiling method then subjected to RAPD and ERIC-PCR. Data gathered can be used to confirm clonal relatedness between isolated colonies which is important for monitoring and tracing the sources of the emerging pathogens.

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Abstract

Vibrio parahaemolyticus is a marine bacterium heavily documented for the presence of Thermostable Direct Haemolysin Gene tdh and Thermostable Direct Haemolysinrelated Haemolysin Gene trh since the 1970s. It is epidemiologically worldwide that spreads through poor sanitation and unhygienic marketplaces in Japan India United States Mexico Indonesia and even Malaysia. This bacterium is often associated with infection via faecal-oral route that causes an array of gastroenteritis symptoms which includes diarrhoea vomiting fever and abdominal pain. Although this bacterium is heat sensitive and can be prevented through hygienic practices it poses a potential public health threat in rural communities and in times of flood disasters. Understanding this bacterium better would enable more efficient emergency care during outbreaks. The purpose of this study is to characterise V. parahaemolyticus carrying tdh and trh gene using molecular typing methods thus determine their clonal relatedness. It is hypothesised that there is specific clonal relatedness among V. parahaemolyticus carrying tdh and trh gene with respect to Random Amplification of Polymorphic DNA RAPD and Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction ERIC-PCR fingerprinting. Using V. parahaemolyticus isolates carrying tdh and trh gene which are collected from previous studies on bloody clam Anadara granosaand Lala Orbicularia orbiculata 33 potential isolates are revived using Tryptic Soy Broth with 3 sodium chloride. DNA is extracted using simple boiling method then subjected to RAPD and ERIC-PCR. Data gathered can be used to confirm clonal relatedness between isolated colonies which is important for monitoring and tracing the sources of the emerging pathogens.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol. 1 Call Number.
AGROVOC Term:	Vibrio
AGROVOC Term:	Vibrio parahaemolyticus
AGROVOC Term:	Gram negative bacteria
AGROVOC Term:	Epidemiology
AGROVOC Term:	Aquatic animal
AGROVOC Term:	Isolation
AGROVOC Term:	Extraction
AGROVOC Term:	Denaturation
AGROVOC Term:	DNA
AGROVOC Term:	Genes
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13054

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