Isolation and characterisation of endophytic fungi producing volatile organic compounds

Rashidah Iberahim, . and Noraziah Mohamad Zin, . and Jacinta Santhanam, . (2011) Isolation and characterisation of endophytic fungi producing volatile organic compounds. [Proceedings Paper]

Endophytic fungi were known to produce volatile organic compounds VOCs with antimicrobial effects. Muscodor albus is one of the novel endophytic fungus that produces a spectrum of biochemical compounds including VOCs which posses inhibitory properties against a wide range of pathogenic plant bacteria and fungi. The objective of this study is to screen for new endophytic fungi that exhibit antimicrobial VOCs from plants in Malay Peninsula. 16 specimens were taken from leaf stem and the root from 10 selected tree species. Isolates taken from three plant species namely Kaduk Medang Sintok and Kayu Manis were found to contain endophytic fungi that showed similarity in terms of the gross morphology smell and antimicrobial activities with M. albus. The antimicrobial assay showed that the three fungi isolated possess low inhibitory effects towards the test fungi but no inhibition towards bacteria. Molecular analysis by PCR techniques showed that the sizes of DNA for all three fungi were similar at around 828 bp. Based on this criteria the fungus was identified as Hypocrea lixii.

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Abstract

Endophytic fungi were known to produce volatile organic compounds VOCs with antimicrobial effects. Muscodor albus is one of the novel endophytic fungus that produces a spectrum of biochemical compounds including VOCs which posses inhibitory properties against a wide range of pathogenic plant bacteria and fungi. The objective of this study is to screen for new endophytic fungi that exhibit antimicrobial VOCs from plants in Malay Peninsula. 16 specimens were taken from leaf stem and the root from 10 selected tree species. Isolates taken from three plant species namely Kaduk Medang Sintok and Kayu Manis were found to contain endophytic fungi that showed similarity in terms of the gross morphology smell and antimicrobial activities with M. albus. The antimicrobial assay showed that the three fungi isolated possess low inhibitory effects towards the test fungi but no inhibition towards bacteria. Molecular analysis by PCR techniques showed that the sizes of DNA for all three fungi were similar at around 828 bp. Based on this criteria the fungus was identified as Hypocrea lixii.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term:	Endophytes
AGROVOC Term:	Fungi
AGROVOC Term:	Isolation of microorganisms
AGROVOC Term:	Identification
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	PCR
AGROVOC Term:	Stems
AGROVOC Term:	Roots
AGROVOC Term:	Piper
AGROVOC Term:	Cinnamomum zeylanicum
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13071

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