A study of cellular morphological changes during biodegradation of methylene blue dye by Sphingomonas paucimobilis using field emission scanning electron microscopy

Teng Tjoon Tow, . and Norhashimah Morad, . and Ogugbue Chimezie Jason, . and Che Noraini Che Hasnam, . and Norli Ismail, . (2011) A study of cellular morphological changes during biodegradation of methylene blue dye by Sphingomonas paucimobilis using field emission scanning electron microscopy. [Proceedings Paper]

The changes in the cellular morphology of Sphingomonas paucimobilis during the biodegradation of Methylene Blue rvIB dye by the bacterium was investigated using the Field EmissionScanning Electron Microscopy FESEM. Dye biodegradation studies were carried out using the bacterium in batch reactors under shaking incubation conditions at room temperature 30C with MB dye solution as the feeding substrate to the microbes. The effects of operational parameters such as pH hydraulic retention time HRT and bacterial dosage on decolourization of the dye were studied while FESEM was used in ascertaining morphological changes due to exposure of the bacterial cells to the cationic dye. Data obtained indicated that pH HRT and bacterial dosage had significant effects on the biodegradation extent of the dye with complete decolourization of dye 1000 mg/L obtained within 5 days of incubation. The rodshaped cells of the bacterium exhibited a crusted morphology and secreted exopolysaccharides EPS on exposure to MB; changes attributed to probablephysiological defence mechanisms to ensure controlled diffusion of dye molecules into cellular structures and to avoid a toxic shock response by the cells. This study thus revealed the ability of S. paucimobilis to degrade high concentrations of dyes and its capability to elicit protective physiological/morphological changes in response to high concentrations of dyes during biodegradation of such toxicants.

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Abstract

The changes in the cellular morphology of Sphingomonas paucimobilis during the biodegradation of Methylene Blue rvIB dye by the bacterium was investigated using the Field EmissionScanning Electron Microscopy FESEM. Dye biodegradation studies were carried out using the bacterium in batch reactors under shaking incubation conditions at room temperature 30C with MB dye solution as the feeding substrate to the microbes. The effects of operational parameters such as pH hydraulic retention time HRT and bacterial dosage on decolourization of the dye were studied while FESEM was used in ascertaining morphological changes due to exposure of the bacterial cells to the cationic dye. Data obtained indicated that pH HRT and bacterial dosage had significant effects on the biodegradation extent of the dye with complete decolourization of dye 1000 mg/L obtained within 5 days of incubation. The rodshaped cells of the bacterium exhibited a crusted morphology and secreted exopolysaccharides EPS on exposure to MB; changes attributed to probablephysiological defence mechanisms to ensure controlled diffusion of dye molecules into cellular structures and to avoid a toxic shock response by the cells. This study thus revealed the ability of S. paucimobilis to degrade high concentrations of dyes and its capability to elicit protective physiological/morphological changes in response to high concentrations of dyes during biodegradation of such toxicants.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term:	Gram negative bacteria
AGROVOC Term:	Dyes
AGROVOC Term:	Biodegradation
AGROVOC Term:	Scanning microscopy
AGROVOC Term:	Microstructure morphology
AGROVOC Term:	Decolorization
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13075

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