Screening and isolation of solvent-producing bacteria for biobutanol production from rice straw

Nurul Atika Mohamad Remli, . and Umi Kalsom Md Shah, . and Suraini Abd Aziz, . and Rosfarizan Mohamad, . (2011) Screening and isolation of solvent-producing bacteria for biobutanol production from rice straw. [Proceedings Paper]

Thirty nine biobutanol producers were successfully isolated from the soil samples Sekinchan from palm oil mill effluent waste in Felda Serting Hilir and hot springs in Selayang and Bentong Malaysia and their ability to produce biobutanol was evaluated. The isolate with the highest biobutanol production was bacterial isolate A3. The bacterial isolate had grown well at 37C in liquid and solid media with complete absence of oxygen. The colonies produced by the bacterium on reinforced clostria medium RCM agar were circular translucent and white in colour. Single cell of the bacterium was straight rods 0.6 to 2m diameter and motile. Identification through 16 S rRNA was performed and the bacterial isolate A3 was classified as Clostridium sporogenes with 99 similarities. From the batch fermentation using rice straw hydrolysate as a carbon source the concentration of biobutanol produced after 120 h of fermentation was 0.3 g/L.

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Abstract

Thirty nine biobutanol producers were successfully isolated from the soil samples Sekinchan from palm oil mill effluent waste in Felda Serting Hilir and hot springs in Selayang and Bentong Malaysia and their ability to produce biobutanol was evaluated. The isolate with the highest biobutanol production was bacterial isolate A3. The bacterial isolate had grown well at 37C in liquid and solid media with complete absence of oxygen. The colonies produced by the bacterium on reinforced clostria medium RCM agar were circular translucent and white in colour. Single cell of the bacterium was straight rods 0.6 to 2m diameter and motile. Identification through 16 S rRNA was performed and the bacterial isolate A3 was classified as Clostridium sporogenes with 99 similarities. From the batch fermentation using rice straw hydrolysate as a carbon source the concentration of biobutanol produced after 120 h of fermentation was 0.3 g/L.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term:	Bacteria
AGROVOC Term:	Isolation of microorganisms
AGROVOC Term:	Soil sampling
AGROVOC Term:	Rice straw
AGROVOC Term:	Agricultural wastes
AGROVOC Term:	Water springs
AGROVOC Term:	Clostridium
AGROVOC Term:	Butanol
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13082

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