Cellulose biodegradation by bacteria isolates from indigenous termite gut

Teoh Teow Chong, . and Philip Koshy, . and Sekaran Muniandy, . and Swee Kelvin Chuan Wei, . and Zainudin Arifin, . and Noni Ajam, . (2011) Cellulose biodegradation by bacteria isolates from indigenous termite gut. [Proceedings Paper]

The bioconversion of lignocellulosic biomass into biofuel has garnered a huge interest among the key players in green technology. The isolation of bacteria with capability to produce key enzymes that can hydrolyze cellulose easily is most wanting. One identified source to isolate potential bacteria with cellulolytic capabilities is the gut of a termite. The termite gut has a rich diversity of microorganisms and some of them have the capability to digest the resilient lignocellulose and convert them into nutrients that can be easily utilized by the termite. Termite specimens were collected in the vicinity of University of Malaya Malaysia. Culturable isolates of aerobic bacteria obtained from soldier termites were screened for their cellulolytic activity using carboxymethylcelluloseCMC medium. The selected cellulolytic isolates were identified using 168 rRNA sequencing and later confirmed using Bergey;s Manual of Determinative Bacteriology. Two cellulolytic aerobic bacteria isolates C4 and C7 were identified and labeled as Bacillus cereus UMC4 and Bacillus subtilis UMC7 respectively. Further studies on the cellulolytic enzymes produced by these bacteria will help us to understand more about the biodegradation of cellulose.

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Abstract

The bioconversion of lignocellulosic biomass into biofuel has garnered a huge interest among the key players in green technology. The isolation of bacteria with capability to produce key enzymes that can hydrolyze cellulose easily is most wanting. One identified source to isolate potential bacteria with cellulolytic capabilities is the gut of a termite. The termite gut has a rich diversity of microorganisms and some of them have the capability to digest the resilient lignocellulose and convert them into nutrients that can be easily utilized by the termite. Termite specimens were collected in the vicinity of University of Malaya Malaysia. Culturable isolates of aerobic bacteria obtained from soldier termites were screened for their cellulolytic activity using carboxymethylcelluloseCMC medium. The selected cellulolytic isolates were identified using 168 rRNA sequencing and later confirmed using Bergey;s Manual of Determinative Bacteriology. Two cellulolytic aerobic bacteria isolates C4 and C7 were identified and labeled as Bacillus cereus UMC4 and Bacillus subtilis UMC7 respectively. Further studies on the cellulolytic enzymes produced by these bacteria will help us to understand more about the biodegradation of cellulose.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term:	Termites
AGROVOC Term:	Isolation of microorganisms
AGROVOC Term:	Biodegradation
AGROVOC Term:	Cellulose
AGROVOC Term:	Bacteria
AGROVOC Term:	Lignocellulose
AGROVOC Term:	Cellulolytic microorganisms
AGROVOC Term:	Bacillus cereus
AGROVOC Term:	Bacillus subtilis
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13083

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