Purification and characterisation of novel antibacterial palm kernel expeller PKE peptide


Citation

Mohd Khan Ayob, . and Tan Yen Nee, . (2011) Purification and characterisation of novel antibacterial palm kernel expeller PKE peptide. [Proceedings Paper]

Abstract

Palm kernel expeller PKE the most useful by-product from oil palm industry has currently being upgraded into value-added palm kernel product. The alcalasehydrolysed peptide from PKE has proven to have antibacterial activity against sporeforming bacteria. We report herein the characterisation of the purified novel antibacterial PKE peptide. Gel filtration column chromatography was used to fractionate and purify the PKE peptide on the basis of differences in molecular size. The purified peptide was estimated to have molecular mass around 2.4 kDa as analysed by tris-tricine SDS-PAGE. The purified peptide at 250 g/mL sufficiently inhibiting the growth of Bacillus cereus. The effect of PKE peptide on the membrane of B. cereus cell was examined by scanning electron microscopy. Our results suggested that PKE peptide exerts antibacterial efficacy was possibly due to the presence of relavent amino acids such as leucine and lysine in the peptide molecule and the leakage of the bacterial membrane.


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Abstract

Palm kernel expeller PKE the most useful by-product from oil palm industry has currently being upgraded into value-added palm kernel product. The alcalasehydrolysed peptide from PKE has proven to have antibacterial activity against sporeforming bacteria. We report herein the characterisation of the purified novel antibacterial PKE peptide. Gel filtration column chromatography was used to fractionate and purify the PKE peptide on the basis of differences in molecular size. The purified peptide was estimated to have molecular mass around 2.4 kDa as analysed by tris-tricine SDS-PAGE. The purified peptide at 250 g/mL sufficiently inhibiting the growth of Bacillus cereus. The effect of PKE peptide on the membrane of B. cereus cell was examined by scanning electron microscopy. Our results suggested that PKE peptide exerts antibacterial efficacy was possibly due to the presence of relavent amino acids such as leucine and lysine in the peptide molecule and the leakage of the bacterial membrane.

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term: Palm kernels
AGROVOC Term: Oil palm
AGROVOC Term: Oil mill byproducts
AGROVOC Term: Purification
AGROVOC Term: Identification
AGROVOC Term: Gel filtration chromatography
AGROVOC Term: Scanning microscopy
AGROVOC Term: Peptides
AGROVOC Term: Bacillus cereus
AGROVOC Term: Bacillus thuringiensis
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:15
URI: http://webagris.upm.edu.my/id/eprint/13084

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