Citation
Haritharan Weloosamy, . and Darah Ibrahim, . (2011) Screening for fungal pectinase producers by submerged fermentation. [Proceedings Paper]
Abstract
Pectinases are a group of hydrolyzing enzymes that catalyzes the breakdown of pectin substances found in plants. The biotechnological potential of pectinolytic enzymes from fungi has drawn a great deal of attention in food processing industries especially in clarification of fruit juices pulp extraction from vegetables and fruits cocoa bean fermentation and also for soluble tea preparation. The present study reports on screening for potential local pectinase producers and effect of culture conditions. A total of 64 isolates were obtained and 43 of them were identified as pectinase producers by qualitative screening using pectin agar medium. Isolate Aspergillus sp. HFD5A-1 which was isolated from a decayed pomelo and was chosen as the best pectinase producer as it produced about 1.36 U/mL pectinase extracellularly. The improved culture conditions initial medium pH of 4.5 incubation temperature of 30C agitation speed of 150 rpm and inoculum size of 1.0 x 107 spores/mL produced about 1.65 U/mL pectinase and 2.82 g/L fungal growth. There was about 21.32 increment after the improvement of cultural condition compared to before improvement.
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Abstract
Pectinases are a group of hydrolyzing enzymes that catalyzes the breakdown of pectin substances found in plants. The biotechnological potential of pectinolytic enzymes from fungi has drawn a great deal of attention in food processing industries especially in clarification of fruit juices pulp extraction from vegetables and fruits cocoa bean fermentation and also for soluble tea preparation. The present study reports on screening for potential local pectinase producers and effect of culture conditions. A total of 64 isolates were obtained and 43 of them were identified as pectinase producers by qualitative screening using pectin agar medium. Isolate Aspergillus sp. HFD5A-1 which was isolated from a decayed pomelo and was chosen as the best pectinase producer as it produced about 1.36 U/mL pectinase extracellularly. The improved culture conditions initial medium pH of 4.5 incubation temperature of 30C agitation speed of 150 rpm and inoculum size of 1.0 x 107 spores/mL produced about 1.65 U/mL pectinase and 2.82 g/L fungal growth. There was about 21.32 increment after the improvement of cultural condition compared to before improvement.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number. |
| AGROVOC Term: | Pectinase |
| AGROVOC Term: | Pectins |
| AGROVOC Term: | Fungi |
| AGROVOC Term: | Enzymatic hydrolysis |
| AGROVOC Term: | Isolation of microorganisms |
| AGROVOC Term: | Screening tests |
| AGROVOC Term: | Aspergillus |
| AGROVOC Term: | Protein content |
| AGROVOC Term: | Food processing |
| AGROVOC Term: | Food industry |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13090 |
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