Growth inhibitory response of non-candida albicans candida NCAC species to Brucea javanica l. seeds extract

Wan Himratul Aznita Wan H., . and Mohd AI Faisal N., . and Fathilah A. R., . (2011) Growth inhibitory response of non-candida albicans candida NCAC species to Brucea javanica l. seeds extract. [Proceedings Paper]

Previous studies have shown that extracts from seeds of Brucea javanica L. possess inhibitory effect on Candida albicans suggesting its potential role in controlling an overgrowth of Candida species. The non-Candida albicans Candida NCAC species have recently been associated with oral candidiasis due to immunosuppressive effect of antifungal agents on resistant hosts. This study aims to evaluate the growth inhibitory effect of B. javanica seeds extract based on the changes in patterns of growth profiles of Candida species. Four Candida species Candida glabrata ATCC 90030 Candida krusei ATCC 14243 Candida lusitaniae ATCC 64125 and Candida parapsilosis ATCC 22019 were grown in four different Yeast Peptone Dextrose broths; without the extract normal and with the extract at respective concentrations 1 3 and 6 mg/mL. The extract was first prepared by decoction and the sub-minimal inhibitory concentrations were used. The reproducible growth curves of different Candida species were distinguished by measuring the changes of specific growth rate -value and doubling time g-value. These values in the presence of extract were computed as percentage in the optical density OD relative to that of the total cells suspension in the absence of extract. Positive control was 0.12 w/v chlorhexidine CHX. Based on parameters of the growth curves the duration of the lag phase was strongly affected by the presence of the extract. Of four C. parapsilosis and C. krusei showed the highest reduction of -values at 1 mg/mL with 90.8 and 88.7 respectively. At a high concentration the IJand g-values of the growth curves were significantly reduced by more than 50 p0.05. The g-values of C. glabrata however were slightly accelerated and vary. The turbidimetric growth assay confirmed the inhibitory effects of B. javanica extract towards these NCAC species and may be considered as a promising candidate for the development of antifungal agent in oral health products.

Full text available from:

Abstract

Previous studies have shown that extracts from seeds of Brucea javanica L. possess inhibitory effect on Candida albicans suggesting its potential role in controlling an overgrowth of Candida species. The non-Candida albicans Candida NCAC species have recently been associated with oral candidiasis due to immunosuppressive effect of antifungal agents on resistant hosts. This study aims to evaluate the growth inhibitory effect of B. javanica seeds extract based on the changes in patterns of growth profiles of Candida species. Four Candida species Candida glabrata ATCC 90030 Candida krusei ATCC 14243 Candida lusitaniae ATCC 64125 and Candida parapsilosis ATCC 22019 were grown in four different Yeast Peptone Dextrose broths; without the extract normal and with the extract at respective concentrations 1 3 and 6 mg/mL. The extract was first prepared by decoction and the sub-minimal inhibitory concentrations were used. The reproducible growth curves of different Candida species were distinguished by measuring the changes of specific growth rate -value and doubling time g-value. These values in the presence of extract were computed as percentage in the optical density OD relative to that of the total cells suspension in the absence of extract. Positive control was 0.12 w/v chlorhexidine CHX. Based on parameters of the growth curves the duration of the lag phase was strongly affected by the presence of the extract. Of four C. parapsilosis and C. krusei showed the highest reduction of -values at 1 mg/mL with 90.8 and 88.7 respectively. At a high concentration the IJand g-values of the growth curves were significantly reduced by more than 50 p0.05. The g-values of C. glabrata however were slightly accelerated and vary. The turbidimetric growth assay confirmed the inhibitory effects of B. javanica extract towards these NCAC species and may be considered as a promising candidate for the development of antifungal agent in oral health products.

[error in script]

Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QR22 M3I61 2011 vol.2 Call Number.
AGROVOC Term:	Simaroubaceae
AGROVOC Term:	Seeds
AGROVOC Term:	Extracts
AGROVOC Term:	Inhibition
AGROVOC Term:	Candida glabrata
AGROVOC Term:	Candida parapsilosis
AGROVOC Term:	Fungicidal properties
AGROVOC Term:	Antifungal agents
AGROVOC Term:	Immunosuppressive agents
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13102

Actions (login required)

View Item