Citation
McRae J. M., . and Yang Q., . and Crawford R. J., . and Palombo E. A., . (2010) Antimicrobial compounds from Australian traditional medicines. [Proceedings Paper]
Abstract
The leaves of the tropical tree Planchonia careya Lecythidaceae are used traditionally in the treatment of wounds and ulcers by indigenous communities in northern Australia. The purpose of this investigation was to isolate and identify some antibacterial compounds from the leaves of P. careya to validate the use of this species as a wound healing remedy and also to evaluate the isolated active compounds for potential use as chemotherapeutic or topical antibacterial agents. The comprising compounds of the crude aqueous and methanol leaf extracts were separated using activity-guided fractionation and the structures of the isolated antibacterial compounds were elucidated using nuclear magnetic resonance NMR spectroscopy and mass spectrometry. The biological activity of each compound was assessed using minimum inhibitory concentration MIC assays against a range of bacteria and with MTT cell viability assays against monkey kidney epithelial MA104 cells Two known antibacterial compounds gallocatechin-4-8-gallocatechin 1 and -gallocatechin 2 were isolated from the aqueous extract. The methanol extract yielded 9 S-hydroxyoctadeca 10E 12Z 15Z trienoic acid 3 9S-hydroxyoctadeca-1 OE 12l-dienoic acid 4 2a 19a-dihydroxyurs-12-en-28-oic acid 5 and a mixture of the cis and trans isomers of 3-O-p-coumaroyl-2a 19a-dihydroxyurs-12-en-28-oic acid 6ab. The mixture of 6ab demonstrated the greatest antibacterial activity and prokaryotic selectivity of the isolated compounds with a MIC against vancomycin resistant enterococci VRE of 59 g/mL. The isolation of known antibacterial compounds 1-5 as well as 6ab from the crude leaf extracts of P. careya validates the use of this species as a treatment for sores and ulcers.
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Abstract
The leaves of the tropical tree Planchonia careya Lecythidaceae are used traditionally in the treatment of wounds and ulcers by indigenous communities in northern Australia. The purpose of this investigation was to isolate and identify some antibacterial compounds from the leaves of P. careya to validate the use of this species as a wound healing remedy and also to evaluate the isolated active compounds for potential use as chemotherapeutic or topical antibacterial agents. The comprising compounds of the crude aqueous and methanol leaf extracts were separated using activity-guided fractionation and the structures of the isolated antibacterial compounds were elucidated using nuclear magnetic resonance NMR spectroscopy and mass spectrometry. The biological activity of each compound was assessed using minimum inhibitory concentration MIC assays against a range of bacteria and with MTT cell viability assays against monkey kidney epithelial MA104 cells Two known antibacterial compounds gallocatechin-4-8-gallocatechin 1 and -gallocatechin 2 were isolated from the aqueous extract. The methanol extract yielded 9 S-hydroxyoctadeca 10E 12Z 15Z trienoic acid 3 9S-hydroxyoctadeca-1 OE 12l-dienoic acid 4 2a 19a-dihydroxyurs-12-en-28-oic acid 5 and a mixture of the cis and trans isomers of 3-O-p-coumaroyl-2a 19a-dihydroxyurs-12-en-28-oic acid 6ab. The mixture of 6ab demonstrated the greatest antibacterial activity and prokaryotic selectivity of the isolated compounds with a MIC against vancomycin resistant enterococci VRE of 59 g/mL. The isolation of known antibacterial compounds 1-5 as well as 6ab from the crude leaf extracts of P. careya validates the use of this species as a treatment for sores and ulcers.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number. |
| AGROVOC Term: | Traditional medicines |
| AGROVOC Term: | Lecythidaceae |
| AGROVOC Term: | Leaves |
| AGROVOC Term: | Isolation in chemical analysis |
| AGROVOC Term: | Identification |
| AGROVOC Term: | Nuclear magnetic resonance spectroscopy |
| AGROVOC Term: | Mass spectrometry |
| AGROVOC Term: | Leaf extracts |
| AGROVOC Term: | Antimicrobial properties |
| AGROVOC Term: | Antibacterial agents |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13146 |
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