Citation
Zin N. M., . and Norazli G., . (2010) Biological promise of bioactive endophytic streptomyces from ethnobotanical plants of the Malay Peninsula. [Proceedings Paper]
Abstract
Endophytic streptomycetes have been isolated and identified from ethno botanical plants of the Malay Peninsula. At least six plants species from the eastern and southern part harbored seven endophytic streptomycetes. The isolates were cultured on Starch casein agar supplemented with the antibiotic nalidixic acid and anti fungal Cycloheximide and Nystatin. All isolates were Gram positive with branching filaments. All isolates as viewed by scanning electron microscopy have small hyphae some produce typical barrel-shaped spores in culture and each has some unique hyphal surface structures. Molecular biological studies on the 16S rRNA gene sequence of each isolate revealed that SUK 01 SUK 02 and SUK 03 were 96-98 similar while SUK 04 5 and 06 were 98 similar to Streptomycetes in the Gene Bank. SUK 02 was antagonistic to Pseudomonas aeruginosa ATCC 27853 Enterococus fecalis and Pleisiomonas shigelloide. Nevertheless SUK 06 was inhibitory to Pseudomonas aeruginosa ATCC 27853 Staphylococus aureus ATCC 25923 Pleisiomonas shigelloides Bacillus subtilis MRSA ATCC 700699 and B. cereus ATCC 6464. The anti bacterial activity of SUK 06 was extractable in various organic solvents and three fractions were acquired. The MIC values for each fraction A Band C was 0.075 1.25 and 0.625 mg/ml respectively.
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Abstract
Endophytic streptomycetes have been isolated and identified from ethno botanical plants of the Malay Peninsula. At least six plants species from the eastern and southern part harbored seven endophytic streptomycetes. The isolates were cultured on Starch casein agar supplemented with the antibiotic nalidixic acid and anti fungal Cycloheximide and Nystatin. All isolates were Gram positive with branching filaments. All isolates as viewed by scanning electron microscopy have small hyphae some produce typical barrel-shaped spores in culture and each has some unique hyphal surface structures. Molecular biological studies on the 16S rRNA gene sequence of each isolate revealed that SUK 01 SUK 02 and SUK 03 were 96-98 similar while SUK 04 5 and 06 were 98 similar to Streptomycetes in the Gene Bank. SUK 02 was antagonistic to Pseudomonas aeruginosa ATCC 27853 Enterococus fecalis and Pleisiomonas shigelloide. Nevertheless SUK 06 was inhibitory to Pseudomonas aeruginosa ATCC 27853 Staphylococus aureus ATCC 25923 Pleisiomonas shigelloides Bacillus subtilis MRSA ATCC 700699 and B. cereus ATCC 6464. The anti bacterial activity of SUK 06 was extractable in various organic solvents and three fractions were acquired. The MIC values for each fraction A Band C was 0.075 1.25 and 0.625 mg/ml respectively.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number. |
| AGROVOC Term: | Ethnobotany |
| AGROVOC Term: | Plants |
| AGROVOC Term: | Medicinal plants |
| AGROVOC Term: | Molecular biology |
| AGROVOC Term: | Isolation of microorganisms |
| AGROVOC Term: | Scanning microscopy |
| AGROVOC Term: | Streptomyces |
| AGROVOC Term: | Pathogenic bacteria |
| AGROVOC Term: | Gene sequence |
| AGROVOC Term: | Antifungal agents |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13161 |
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