Phytochemical analysis and antimicrobial screening of selected medicinal herbs used in Malaysian diet

Vikneswaran V., . and Jegathambigai R., . and Sasidharan S., . (2010) Phytochemical analysis and antimicrobial screening of selected medicinal herbs used in Malaysian diet. [Proceedings Paper]

Drug development to combat infectious disease has gain popularity in many researches at present time. The undesirable side effects of certain antibiotics and the emergence of previously uncommon infections have forced scientists to look for new antimicrobial substances from various sources like medicinal plants. Antimicrobial screening and phytochemical analysis was done for seven different plant spcies including Cymbopogon citratus Lemon grass Citrus hystrix Kaffir lime leaf Pandanus amaryllifolius Pandan Murraya koenigii Curry leaf Allium ascalonicum Spring onion Apium graveolens Celery and Mentha spicata Pudina ethanolic extract were analyzed initially. Disc diffusion assay was done by using four different bacterial strain Staphylococcus aereus Escherichia coli Shigella sonnei and Salmonella choleraciuis. Further investigation including Minimum Inhibitory Concentration MIC and Thin Layer Chromatography TLC was determined for positive sample in antimicrobial screening Phytochemical analysis indicated that glycoside was present in ethanolic extract of A graveolens and M spicata while flavonoid was present only in C citratus. Tannins and saponins were present in P. amaryllifoluis M koenigii C. hystrix and Aascalonicum while A graveolens only had tannins. Presence of anthroquinone was detected only in ethanolic extract of C.citratus. The antimicrobial screening results of ethanolic extract of C.citratus inhibited growth of only S.aereus bacterial strain with an inhibition zone of 0.870 12cm while the other plants did not show any antimicrobial activity Ethanolic extract of six other samples showed negative effect on all four strains of bacteria. The MIC value of S.aereus against C. citratus is 12.5mg/ml. Hence C. citratus has potential antimicrobial activity which can be further studied for active compound.

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Abstract

Drug development to combat infectious disease has gain popularity in many researches at present time. The undesirable side effects of certain antibiotics and the emergence of previously uncommon infections have forced scientists to look for new antimicrobial substances from various sources like medicinal plants. Antimicrobial screening and phytochemical analysis was done for seven different plant spcies including Cymbopogon citratus Lemon grass Citrus hystrix Kaffir lime leaf Pandanus amaryllifolius Pandan Murraya koenigii Curry leaf Allium ascalonicum Spring onion Apium graveolens Celery and Mentha spicata Pudina ethanolic extract were analyzed initially. Disc diffusion assay was done by using four different bacterial strain Staphylococcus aereus Escherichia coli Shigella sonnei and Salmonella choleraciuis. Further investigation including Minimum Inhibitory Concentration MIC and Thin Layer Chromatography TLC was determined for positive sample in antimicrobial screening Phytochemical analysis indicated that glycoside was present in ethanolic extract of A graveolens and M spicata while flavonoid was present only in C citratus. Tannins and saponins were present in P. amaryllifoluis M koenigii C. hystrix and Aascalonicum while A graveolens only had tannins. Presence of anthroquinone was detected only in ethanolic extract of C.citratus. The antimicrobial screening results of ethanolic extract of C.citratus inhibited growth of only S.aereus bacterial strain with an inhibition zone of 0.870 12cm while the other plants did not show any antimicrobial activity Ethanolic extract of six other samples showed negative effect on all four strains of bacteria. The MIC value of S.aereus against C. citratus is 12.5mg/ml. Hence C. citratus has potential antimicrobial activity which can be further studied for active compound.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number.
AGROVOC Term:	Medicinal plants
AGROVOC Term:	Phytochemistry
AGROVOC Term:	Screening tests
AGROVOC Term:	Cymbopogon citratus
AGROVOC Term:	Lemon grass
AGROVOC Term:	Citrus
AGROVOC Term:	Murraya koenigii
AGROVOC Term:	Pandanus
AGROVOC Term:	Curry leaf tree
AGROVOC Term:	Allium ascalonicum
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:15
URI:	http://webagris.upm.edu.my/id/eprint/13167

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