Isolation of acetic acid bacteria from vinegar and its application for over-production of natural vinegar from pineapple


Citation

M. Ramli, . and N. Othman, . and I. Latif S., . and M. R. Sarmidi, . and R. A. Malek, . and R. Abd Aziz, . and N. Mohd. Noor, . (2010) Isolation of acetic acid bacteria from vinegar and its application for over-production of natural vinegar from pineapple. [Proceedings Paper]

Abstract

Vinegar is one of the oldest known fermentation products. Nowadays vinegar application is not limited for culinary use but also find many applications in medical nutraceutical and cosmeceutical industries. The production of vinegar is carried out either by natural fermentation of alcohol by acetic acid bacteria. The present work focuses on the production of natural vinegar from pineapple using newly isolated bacterial strain. The first part of this study was focused on the isolation of local strains from natural vinegar. Four strains of acetic acid bacteria were isolated and identified using biochemical identification method API 120E and APILAB Plus software V.3.2.2. All strains isolated were found belong to Acetobacter aceti. The most promising isolate was deposited at CEPP culture collection under the name Aeetobacter aceti WICC B-01. The performance of this new strain for acetic acid production was compared to the standard strain A. aceti DSMZ 3508. After 6 days fermentation A. aceti WICC B-01 produced 3.97 acetic acid compared to only 2.95 produced by standard A. aceti DSMZ 3508 under a similar cultivation conditions. Moreover the vinegar produced by the newly isolated strain was characterized by a pleasant fruity pineapple aroma.


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Abstract

Vinegar is one of the oldest known fermentation products. Nowadays vinegar application is not limited for culinary use but also find many applications in medical nutraceutical and cosmeceutical industries. The production of vinegar is carried out either by natural fermentation of alcohol by acetic acid bacteria. The present work focuses on the production of natural vinegar from pineapple using newly isolated bacterial strain. The first part of this study was focused on the isolation of local strains from natural vinegar. Four strains of acetic acid bacteria were isolated and identified using biochemical identification method API 120E and APILAB Plus software V.3.2.2. All strains isolated were found belong to Acetobacter aceti. The most promising isolate was deposited at CEPP culture collection under the name Aeetobacter aceti WICC B-01. The performance of this new strain for acetic acid production was compared to the standard strain A. aceti DSMZ 3508. After 6 days fermentation A. aceti WICC B-01 produced 3.97 acetic acid compared to only 2.95 produced by standard A. aceti DSMZ 3508 under a similar cultivation conditions. Moreover the vinegar produced by the newly isolated strain was characterized by a pleasant fruity pineapple aroma.

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP248.14 I61 2008 Call Number.
AGROVOC Term: Pineapples
AGROVOC Term: Isolation of microorganisms
AGROVOC Term: Fermentation
AGROVOC Term: Acetic acid bacteria
AGROVOC Term: Vinegar
AGROVOC Term: morphology
AGROVOC Term: Physiology
AGROVOC Term: biochemical compounds
AGROVOC Term: Mineral content
Geographical Term: MALAYSIA
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 05:15
URI: http://webagris.upm.edu.my/id/eprint/13176

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