Citation
Yeoh K. A., . and Tan Y. C., . and Wong M. Y., . and Othman A., . and Ho C. L., . and Meon S., . (2013) Transcriptomic markers for Ganoderma resistance. [Proceedings Paper]
Abstract
Basal stem rot BSR is the most serious fungal disease in oil palm. BSR can be detected by several methods such as hyperspectral imaging that can help to identify severely infected palms detection of fungal ergosterol and fungal DNA or proteins by polymerase chain reaction PCR or polyclonal antibodies. Although oil palms from different genetic origins have been reported to be tolerant or resistant to BSR the genes involved in the tolerance and resistance of these oil palms against pathogenic Ganoderma are unknown. Transcrips that are involved in the defence response of oil palm may have the potential to be developed as biomarkers for early detection of Ganoderma infection and also for the screening of Ganoderma-resistant oil palm planting materials. To identify the transcripts that are associated with the defence response of oil palm to G. boninense we have profiled the transcripts in oil palm roots infected by G. boninense with cDNA microarray and high throughput RNA-sequencing approaches. Our findings revealed many genes that were differentially regulated in oil palm roots infected by G. boninense compared to uninfected oil palm roots. Among these genes were genes related to defense and stresses such as putative chitinases glucanases Bowman-Birk serine protease inhibitors defensin dehydrin pathogenesis-related-1 protein and type 2 ribosome inactivating protein. The transcript abundance of these genes were verified in infected oil palm roots and also analysed in leaf samples to identify potential biomarkers for early detection of BSR. The usefulness of these markers will be discussed.
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Abstract
Basal stem rot BSR is the most serious fungal disease in oil palm. BSR can be detected by several methods such as hyperspectral imaging that can help to identify severely infected palms detection of fungal ergosterol and fungal DNA or proteins by polymerase chain reaction PCR or polyclonal antibodies. Although oil palms from different genetic origins have been reported to be tolerant or resistant to BSR the genes involved in the tolerance and resistance of these oil palms against pathogenic Ganoderma are unknown. Transcrips that are involved in the defence response of oil palm may have the potential to be developed as biomarkers for early detection of Ganoderma infection and also for the screening of Ganoderma-resistant oil palm planting materials. To identify the transcripts that are associated with the defence response of oil palm to G. boninense we have profiled the transcripts in oil palm roots infected by G. boninense with cDNA microarray and high throughput RNA-sequencing approaches. Our findings revealed many genes that were differentially regulated in oil palm roots infected by G. boninense compared to uninfected oil palm roots. Among these genes were genes related to defense and stresses such as putative chitinases glucanases Bowman-Birk serine protease inhibitors defensin dehydrin pathogenesis-related-1 protein and type 2 ribosome inactivating protein. The transcript abundance of these genes were verified in infected oil palm roots and also analysed in leaf samples to identify potential biomarkers for early detection of BSR. The usefulness of these markers will be discussed.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. SB608 O27M939 2013 Call Number. |
| AGROVOC Term: | Oil palm |
| AGROVOC Term: | Plant disease control |
| AGROVOC Term: | Ganoderma |
| AGROVOC Term: | Fungal diseases |
| AGROVOC Term: | Rots |
| AGROVOC Term: | Biological markers molecular |
| AGROVOC Term: | Genes |
| AGROVOC Term: | Gene expression |
| AGROVOC Term: | Defense response |
| AGROVOC Term: | Pathogenesis |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13207 |
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