Citation
Ramie Moslim, . and Zulkefli Masijan, . and Norman Kamarudin, . (2013) Impact of spores of entomopathogenic fungi transmitted by termite worker Coptotermes curvignathus on mortality of healthy termite colony. [Proceedings Paper]
Abstract
The impact of spores of seven isolates of entomopathogenic fungi Metarhizium anisopliae MaTGK2 MaSE MaST MaBP and Baeuveria bassiana BbF1 BbF5 BbF10 transmitted by termite workers Coptotermes curvignathus on mortality of healthy termite colony was evaluated in the laboratory. Prior to inoculation the termite workers were introduced into a petri dish containing a filter paper stained with 0.1 g Nile blue solution. The workers were then individually inoculated by dropping of 20 l spore solutions at concentrations of 10 10� and 10�· spores ml-. The dish was kept in an incubator at 20C and 80-90 RH for overnight. Fifteen workers with blue stain in the gut were transferred into a new petri dish containing of 15 healthy termite workers. The results showed that the inoculated workers could transmit the fungal spores and causing death to the healthy termite colony. As expected the mortality of the inoculated termites was always higher than the mortality of the healthy termite colony in all three rates of spore concentrations. At 10 days after inoculation DAI and at the highest inoculation rate of 10�· spores ml- six isolates killed more than 50 of the inoculated termites. At this stage the transmitted spores of two isolates MaTGK2 MaSE killed the highest numbers the healthy termites 40 followed by the isolates BbF5 and BbF10 35 then MaST 25 MaBP 20 and BbF1 15. The percentages of dead of healthy workers infected by the transmitted spores were low ranging from 0 to the highest level of 11.1 which was caused by the isolate BbF5.
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Abstract
The impact of spores of seven isolates of entomopathogenic fungi Metarhizium anisopliae MaTGK2 MaSE MaST MaBP and Baeuveria bassiana BbF1 BbF5 BbF10 transmitted by termite workers Coptotermes curvignathus on mortality of healthy termite colony was evaluated in the laboratory. Prior to inoculation the termite workers were introduced into a petri dish containing a filter paper stained with 0.1 g Nile blue solution. The workers were then individually inoculated by dropping of 20 l spore solutions at concentrations of 10 10� and 10�· spores ml-. The dish was kept in an incubator at 20C and 80-90 RH for overnight. Fifteen workers with blue stain in the gut were transferred into a new petri dish containing of 15 healthy termite workers. The results showed that the inoculated workers could transmit the fungal spores and causing death to the healthy termite colony. As expected the mortality of the inoculated termites was always higher than the mortality of the healthy termite colony in all three rates of spore concentrations. At 10 days after inoculation DAI and at the highest inoculation rate of 10�· spores ml- six isolates killed more than 50 of the inoculated termites. At this stage the transmitted spores of two isolates MaTGK2 MaSE killed the highest numbers the healthy termites 40 followed by the isolates BbF5 and BbF10 35 then MaST 25 MaBP 20 and BbF1 15. The percentages of dead of healthy workers infected by the transmitted spores were low ranging from 0 to the highest level of 11.1 which was caused by the isolate BbF5.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. SB608 O27M939 2013 Call Number. |
| AGROVOC Term: | Oil palm |
| AGROVOC Term: | Plant disease control |
| AGROVOC Term: | Entomopathogenic fungi |
| AGROVOC Term: | Spores |
| AGROVOC Term: | Coptotermes |
| AGROVOC Term: | Termites |
| AGROVOC Term: | Beauveria bassiana |
| AGROVOC Term: | Metarhizium anisopliae |
| AGROVOC Term: | Mortality |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/13230 |
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