Citation
Normah M. N., . and Nor Asmah H., . and Aziah M. Y., . (2003) Micropropagation studies on Shorea leprosula. [Proceedings Paper]
Abstract
Shorea leprosula from the Dipterocarpaceae family is widely found in the natural forests of Peninsular Malaysia Sumatra and Borneo. It can be propagated by cuttings and seed. In this study shoot explants from one-year-old saplings raised in the nursery were used as one of the initial sources of explants for sterilization experiments. Fungicide Benlate antibiotic streptomycin and antioxidant ascorbic acid were used in pretreatment prior to sterilization to reduce contamination and browning. Mature seeds were sterilized and germinated in vitro using Woody Plant Medium WPM. The shoots and cotyledonary nodes of these in vitro seedlings were used in multiplication study. It was observed that cotyledonary node explants gave the highest number of shoots with 4.4 M BAP 6-benzylaminopurine treatment.
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Abstract
Shorea leprosula from the Dipterocarpaceae family is widely found in the natural forests of Peninsular Malaysia Sumatra and Borneo. It can be propagated by cuttings and seed. In this study shoot explants from one-year-old saplings raised in the nursery were used as one of the initial sources of explants for sterilization experiments. Fungicide Benlate antibiotic streptomycin and antioxidant ascorbic acid were used in pretreatment prior to sterilization to reduce contamination and browning. Mature seeds were sterilized and germinated in vitro using Woody Plant Medium WPM. The shoots and cotyledonary nodes of these in vitro seedlings were used in multiplication study. It was observed that cotyledonary node explants gave the highest number of shoots with 4.4 M BAP 6-benzylaminopurine treatment.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK495 D564R859 2002 Call Number. |
| AGROVOC Term: | Shorea |
| AGROVOC Term: | Dipterocarpaceae |
| AGROVOC Term: | Dipterocarps |
| AGROVOC Term: | Timber trees |
| AGROVOC Term: | Plant propagation |
| AGROVOC Term: | Micropropagation |
| AGROVOC Term: | In vitro culture |
| AGROVOC Term: | Tissue culture |
| AGROVOC Term: | Explants |
| AGROVOC Term: | Cotyledons |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:16 |
| URI: | http://webagris.upm.edu.my/id/eprint/13403 |
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