Citation
Mohd Farid A., . and Mohd Rosli H., . and Lee S. S., . (2003) Characterization of Colletotrichum associated with anthracnose of Dipterocarp seedlings from two local nurseries. [Proceedings Paper]
Abstract
Isolation of fungi was carried out from dipterocarp seedling leaves affected by anthracnose. The seedlings were obtained from two local nurseries. The causal organisms were identified based on cultural macroscopic and microscopic characters. All cultures yielding Colletotrichum spp. were studied in detail. Conidia shape and dimension septation presence of setae perithecia shape of appressoria and growth rate were recorded for identification to species level. Sequencing of the internal transcribed spacer region ITS of ribosomal DNA was also conducted to elucidate the molecular variation of the Colletotrichum isolates. This technique was used to determine the genetic variation of the pathogen and its relatedness among the group members obtained from different dipterocarp hosts. Depending on the host species anthracnose may produce symptoms that are generally restricted to the leaves or may produce dieback and canker-like symptoms on shoots and branches. Symptoms on the different dipterocarp hosts surveyed in this study were almost similar with little variation. Leaf spots and lesions were the main symptoms found on the infected seedlings. Cultural and morphological studies ascertained C. gloeosporioides as the main species associated with the diseased leaves. This fungus was widely distributed in both the local nurseries irrespective of host species. This was confirmed by results of the cloning and sequencing of the ITS of each isolate.
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Abstract
Isolation of fungi was carried out from dipterocarp seedling leaves affected by anthracnose. The seedlings were obtained from two local nurseries. The causal organisms were identified based on cultural macroscopic and microscopic characters. All cultures yielding Colletotrichum spp. were studied in detail. Conidia shape and dimension septation presence of setae perithecia shape of appressoria and growth rate were recorded for identification to species level. Sequencing of the internal transcribed spacer region ITS of ribosomal DNA was also conducted to elucidate the molecular variation of the Colletotrichum isolates. This technique was used to determine the genetic variation of the pathogen and its relatedness among the group members obtained from different dipterocarp hosts. Depending on the host species anthracnose may produce symptoms that are generally restricted to the leaves or may produce dieback and canker-like symptoms on shoots and branches. Symptoms on the different dipterocarp hosts surveyed in this study were almost similar with little variation. Leaf spots and lesions were the main symptoms found on the infected seedlings. Cultural and morphological studies ascertained C. gloeosporioides as the main species associated with the diseased leaves. This fungus was widely distributed in both the local nurseries irrespective of host species. This was confirmed by results of the cloning and sequencing of the ITS of each isolate.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK495 D564R859 2002 Call Number. |
| AGROVOC Term: | Dipterocarps |
| AGROVOC Term: | Isolation of microorganisms |
| AGROVOC Term: | Seedlings |
| AGROVOC Term: | Leaves |
| AGROVOC Term: | Plant nurseries |
| AGROVOC Term: | Colletotrichum |
| AGROVOC Term: | Fungi |
| AGROVOC Term: | Anthracnosis |
| AGROVOC Term: | Plant diseases |
| AGROVOC Term: | Hosts |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:16 |
| URI: | http://webagris.upm.edu.my/id/eprint/13483 |
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