Hypocholesterolemic effects of different concentration of Tinospora crispa aqueous extract Patawali on rabbits fed with high cholesterol diet

Zulkhairi H. A., . and Mohd Kamal N. H., . and Ihsan S. K., . and Rasadah M. A., . and Zamree M. S., . and Shahidan M. A., . and Kamilah K. A. K., . and Mohd Farhan A. R., . (2013) Hypocholesterolemic effects of different concentration of Tinospora crispa aqueous extract Patawali on rabbits fed with high cholesterol diet. [Proceedings Paper]

This study was conducted to determine the amount of Tinospora crispa aqueous extract TCAE required for optimum hypocholesterolemic effect against hypercholesterolemic induced rabbits. For ten weeks TCAE at a concentration ranging fom 200 to 600 mg/kg were introduce to forty two male New Zealand White Rabbits randomly assigned into six groups according to their diet type vis a vis normal diet NC 0.5 high cholesterol diet PC 0.5 high cholesterol diet10 mg/kg simvastatin SC 0.5 high cholesterol diet200 mg/kg TCAE TCAE200 0.5 high cholesterol diet450 mg/kg TCAE TCAE450 and 0.5 high cholesterol diet600 mg/kg TCAE TCAE600. Blood samples were withdrawn at week a w0 and 10 w10. Supplementation of 0.5 high cholesterol diet elevated the TC and LDL as well as significantly raised p0.05 the level of liver enzymes compared to the normal control group. Results revealed that all groups supplemented with T.crispa had a significantly higher p0.05 high density lipoprotein HDL level compared to PC at w10 Group. TCAE450 had a significantly lower p0.05 LDL level and total cholesterol TC level compared to PC at w10. All groups supplemented with T.crispa were able to prevent the increment of liver enzymes: gammaglutamyl transferase as well as increased the level of antioxidant defence enzymes: superoxide dismutase and glutathione peroxidase compared to positive control group. This study reaffirmed the potential of 450 mg/kg of TCAE as appropriate concentration to be used in further formulation and product development studies. In general it is suggested that the enhanced serum HOL decrease in serum LOL as well as total cholesterol and increase in antioxidant defence enzymes level may be the possible underlying mechanisms of antiatherogenic effect of T.crispa although it is not comparable with simvastatin.

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Abstract

This study was conducted to determine the amount of Tinospora crispa aqueous extract TCAE required for optimum hypocholesterolemic effect against hypercholesterolemic induced rabbits. For ten weeks TCAE at a concentration ranging fom 200 to 600 mg/kg were introduce to forty two male New Zealand White Rabbits randomly assigned into six groups according to their diet type vis a vis normal diet NC 0.5 high cholesterol diet PC 0.5 high cholesterol diet10 mg/kg simvastatin SC 0.5 high cholesterol diet200 mg/kg TCAE TCAE200 0.5 high cholesterol diet450 mg/kg TCAE TCAE450 and 0.5 high cholesterol diet600 mg/kg TCAE TCAE600. Blood samples were withdrawn at week a w0 and 10 w10. Supplementation of 0.5 high cholesterol diet elevated the TC and LDL as well as significantly raised p0.05 the level of liver enzymes compared to the normal control group. Results revealed that all groups supplemented with T.crispa had a significantly higher p0.05 high density lipoprotein HDL level compared to PC at w10 Group. TCAE450 had a significantly lower p0.05 LDL level and total cholesterol TC level compared to PC at w10. All groups supplemented with T.crispa were able to prevent the increment of liver enzymes: gammaglutamyl transferase as well as increased the level of antioxidant defence enzymes: superoxide dismutase and glutathione peroxidase compared to positive control group. This study reaffirmed the potential of 450 mg/kg of TCAE as appropriate concentration to be used in further formulation and product development studies. In general it is suggested that the enhanced serum HOL decrease in serum LOL as well as total cholesterol and increase in antioxidant defence enzymes level may be the possible underlying mechanisms of antiatherogenic effect of T.crispa although it is not comparable with simvastatin.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK99 M3S471 2012 Call Number.
AGROVOC Term:	Tinospora
AGROVOC Term:	Plant extracts
AGROVOC Term:	Clinical experimentation
AGROVOC Term:	Blood sampling
AGROVOC Term:	Rabbits
AGROVOC Term:	Cholesterol
AGROVOC Term:	Lipoproteins
AGROVOC Term:	Antioxidants
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:16
URI:	http://webagris.upm.edu.my/id/eprint/13659

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