Citation
Christina V. S., . and Menaka G., . and Peter G. A., . (2011) Antifungal effect of chitosan on the mycelial growth of Ganoderma boninense Basal Stem Rot BSR pathogen of oil palm in vitro. [Proceedings Paper]
Abstract
Ganoderma boninense isolates GBB 7 GBB 18 GBLS and GBU were collected from BSR infected oil palm and was identified microscopically while pure mycelia culture was confirmed by PCR techniques. GBLS is the most aggressive isolate screened through pathogenicity test using rubber wood block inoculation technique. After 20 days all of the concentrations 0.5 1 1.5 2.0 w/v significantly reduced in vitro mycelial growth of GBLS when compared with control treatment. Chitosan exhibited a fungistatic effect on mycelial growth of G. boninense and markedly reduced radial growth with increased concentration. The highest inhibition of radial growth PIRG was observed with chitosan at 2.0 w/v. The germination of G. boninense basidiospores treated with 1000 and10000 ppm of chitosan were affected significantly after 14 days incubation period. Chitosan also caused morphological changes in G. boninense mycelium including the occurrence of small vesicles due to coagulation of fungal cytoplasm and formation of excessive abnormal hyphal branching at higher concentrations.
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Abstract
Ganoderma boninense isolates GBB 7 GBB 18 GBLS and GBU were collected from BSR infected oil palm and was identified microscopically while pure mycelia culture was confirmed by PCR techniques. GBLS is the most aggressive isolate screened through pathogenicity test using rubber wood block inoculation technique. After 20 days all of the concentrations 0.5 1 1.5 2.0 w/v significantly reduced in vitro mycelial growth of GBLS when compared with control treatment. Chitosan exhibited a fungistatic effect on mycelial growth of G. boninense and markedly reduced radial growth with increased concentration. The highest inhibition of radial growth PIRG was observed with chitosan at 2.0 w/v. The germination of G. boninense basidiospores treated with 1000 and10000 ppm of chitosan were affected significantly after 14 days incubation period. Chitosan also caused morphological changes in G. boninense mycelium including the occurrence of small vesicles due to coagulation of fungal cytoplasm and formation of excessive abnormal hyphal branching at higher concentrations.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. SB608 O27M939 2011 Call Number. |
| AGROVOC Term: | Oil palm |
| AGROVOC Term: | Plant disease control |
| AGROVOC Term: | Biological control |
| AGROVOC Term: | In vitro culture |
| AGROVOC Term: | Isolation of microorganisms |
| AGROVOC Term: | Ganoderma |
| AGROVOC Term: | Pathogens |
| AGROVOC Term: | Chitosan |
| AGROVOC Term: | Spore germination |
| AGROVOC Term: | Fungal morphology |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:16 |
| URI: | http://webagris.upm.edu.my/id/eprint/13672 |
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