Phytochemical fingerprint analysis of Senna alata by high performance thin layer chromatography HPTLC

Nuziah H., . and Zunoliza A., . and Ling S. K., . (2013) Phytochemical fingerprint analysis of Senna alata by high performance thin layer chromatography HPTLC. [Proceedings Paper]

Phytochemical fingerprints play an important role in the quality control of complex herbal medicines. The fingerprints obtained by chromatographic techniques such as high performance thin layer chromatography HPTLC and high performance liquid chromatography HPLC have been strongly recommended for the purpose of quality control in herbal medicines. The TLC and HPTLC have been used in many studies for the identification assay and testing of purity dissolution or content uniformity of raw material and finished products. These applications give an advantage in the simultaneous process for detection of standards and sample in a variety of method detection possibilities including different solvent systems and post-chromatograhic derivatization reagents. Senna alata is an ornamental shrub of Fabaceae family. It is used traditionally for the treatment of haemorrhoids constipation inguinal hernia intestinal parasite syphilis diabetes convulsion heart failure abnormal pain oedema and infections which include ringworm and parasitic skin diseases. The ethanolic and methanolic extracts of this plant have been reported to have antimicrobial and antifungal properties. The antimicrobial activity is associated with the presence of components such as phenols tannins saponins alkaloids steroids flavonoids and carbohydrates. The aim of this study was to optimize the chromatographic conditions which include the extraction parameters for HPTLC fingerprinting purposes as well as to establish a documented procedure for the study of S. alata. The chromatographic fingerprint can be used as a tool for the authentication based on the phytochemical constituents distributed in the species studied. Briefly the dried and powdered leaf of S. alata was separately extracted with eight solvents with different polarities by soaking reflux and sonication. Parameters such as extraction duration and temperature were also studied. The HPTLC was performed on aluminium sheets of silica gel 60 F254 with fluorescent indicator using Camag Linomat 5. Each plate was developed by a combination of solvents with different polarity as the mobile phase. The development tank was presaturated with the selected mobile phase for 10 min before the developing process. Scanning and densitometric analysis was performed at 254 nm and 366 nm using Camag TLC Scanner 3. The chromatogram was then visualized by dipping with various derivatization reagents for detection. Results of the study showed some similarities and differences of phytochemical constituents among the selected extraction solvents. The optimized extraction and chromatographic methods were obtained and can be used for the quality evaluation and standardization purpose of S. alata.

Full text available from:

Abstract

Phytochemical fingerprints play an important role in the quality control of complex herbal medicines. The fingerprints obtained by chromatographic techniques such as high performance thin layer chromatography HPTLC and high performance liquid chromatography HPLC have been strongly recommended for the purpose of quality control in herbal medicines. The TLC and HPTLC have been used in many studies for the identification assay and testing of purity dissolution or content uniformity of raw material and finished products. These applications give an advantage in the simultaneous process for detection of standards and sample in a variety of method detection possibilities including different solvent systems and post-chromatograhic derivatization reagents. Senna alata is an ornamental shrub of Fabaceae family. It is used traditionally for the treatment of haemorrhoids constipation inguinal hernia intestinal parasite syphilis diabetes convulsion heart failure abnormal pain oedema and infections which include ringworm and parasitic skin diseases. The ethanolic and methanolic extracts of this plant have been reported to have antimicrobial and antifungal properties. The antimicrobial activity is associated with the presence of components such as phenols tannins saponins alkaloids steroids flavonoids and carbohydrates. The aim of this study was to optimize the chromatographic conditions which include the extraction parameters for HPTLC fingerprinting purposes as well as to establish a documented procedure for the study of S. alata. The chromatographic fingerprint can be used as a tool for the authentication based on the phytochemical constituents distributed in the species studied. Briefly the dried and powdered leaf of S. alata was separately extracted with eight solvents with different polarities by soaking reflux and sonication. Parameters such as extraction duration and temperature were also studied. The HPTLC was performed on aluminium sheets of silica gel 60 F254 with fluorescent indicator using Camag Linomat 5. Each plate was developed by a combination of solvents with different polarity as the mobile phase. The development tank was presaturated with the selected mobile phase for 10 min before the developing process. Scanning and densitometric analysis was performed at 254 nm and 366 nm using Camag TLC Scanner 3. The chromatogram was then visualized by dipping with various derivatization reagents for detection. Results of the study showed some similarities and differences of phytochemical constituents among the selected extraction solvents. The optimized extraction and chromatographic methods were obtained and can be used for the quality evaluation and standardization purpose of S. alata.

[error in script]

Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK99 M3S471 2012 Call Number.
AGROVOC Term:	Senna alata
AGROVOC Term:	Fabaceae
AGROVOC Term:	Ornamental shrubs
AGROVOC Term:	Extraction in chemical analysis
AGROVOC Term:	High performance liquid chromatography
AGROVOC Term:	Water
AGROVOC Term:	Methanol
AGROVOC Term:	Water
AGROVOC Term:	Kaempferol
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:16
URI:	http://webagris.upm.edu.my/id/eprint/13718

Actions (login required)

View Item