Citation
Rohana S., . and Nik Musa;adah M., . and Mohd Radzi A., . and Azman M., . and Mohamad Jemain M. R., . and Nurdalila Z., . and Abd Rashid L., . and Normaizira H., . (2013) Cytotoxicity and acute toxicity studies of polysaccharide-rich fraction from Morinda citrifolia fruits. [Proceedings Paper]
Abstract
Morinda citrifolia locally known as ;mengkudu; has been traditionally used in the treatment for several diseases. In our study we found that the polysaccharide-rich fraction from M. citrifolia fruits showed antidiabetic effect through the inhibition of carbohydrate digestive enzyme a-amylase activity. Its safety however is yet to be determined thus evaluated through this study. The Sulphorhadamine B SRB assay was used to analyse the cytotoxicity of the polysaccharide-rich fraction-rich fraction against normal human liver WRL-68 and green monkey kidney Vero cell lines. The polysaccharide-rich fraction;s registered an IC‚o value of more than 1000 g/ml against both cell lines indicating absence of cytotoxicity. Ensuing acute toxicity test was conducted according to the Organisation for Economic Corporation and Development OECD 420 2001 to confirm the in vitro findings. Neither mortality nor signs of toxicity were observed amongst female rats loaded with the polysacharide-rich fraction via gavage at single dose of 2 g/kg observed either immediately or during 2 weeks duration showed. The estimated LO so value was more than 2 g/kg. The body and organ weights were not affected by the treatment and macroscopic analysis displayed no significant changes in colour and texture of the liver. Similarly biochemistry analysis of the liver function showed no significant differences of serum alanine transaminase ALT and aspartate transaminase AST levels as compared to control group. These results indicated that the polysaccharide-rich fraction did not produce cytotoxicity and acute toxicity.
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Abstract
Morinda citrifolia locally known as ;mengkudu; has been traditionally used in the treatment for several diseases. In our study we found that the polysaccharide-rich fraction from M. citrifolia fruits showed antidiabetic effect through the inhibition of carbohydrate digestive enzyme a-amylase activity. Its safety however is yet to be determined thus evaluated through this study. The Sulphorhadamine B SRB assay was used to analyse the cytotoxicity of the polysaccharide-rich fraction-rich fraction against normal human liver WRL-68 and green monkey kidney Vero cell lines. The polysaccharide-rich fraction;s registered an IC‚o value of more than 1000 g/ml against both cell lines indicating absence of cytotoxicity. Ensuing acute toxicity test was conducted according to the Organisation for Economic Corporation and Development OECD 420 2001 to confirm the in vitro findings. Neither mortality nor signs of toxicity were observed amongst female rats loaded with the polysacharide-rich fraction via gavage at single dose of 2 g/kg observed either immediately or during 2 weeks duration showed. The estimated LO so value was more than 2 g/kg. The body and organ weights were not affected by the treatment and macroscopic analysis displayed no significant changes in colour and texture of the liver. Similarly biochemistry analysis of the liver function showed no significant differences of serum alanine transaminase ALT and aspartate transaminase AST levels as compared to control group. These results indicated that the polysaccharide-rich fraction did not produce cytotoxicity and acute toxicity.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QK99 M3S471 2012 Call Number. |
| AGROVOC Term: | Morinda |
| AGROVOC Term: | Morinda citrifolia |
| AGROVOC Term: | Rubiaceae |
| AGROVOC Term: | Medicinal plants |
| AGROVOC Term: | Microbiological analysis |
| AGROVOC Term: | Extraction |
| AGROVOC Term: | Cell culture |
| AGROVOC Term: | Fruits |
| AGROVOC Term: | Cytotoxicity |
| AGROVOC Term: | Acute toxicity |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:16 |
| URI: | http://webagris.upm.edu.my/id/eprint/13726 |
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