Gene expression profiling in Nipah virus infected cell Mcf-7 using DNA microarray

Suzana M. I., . and Zulkeflie Z., . and Sharifah S. H., . and Mohd Ali A. R., . (2005) Gene expression profiling in Nipah virus infected cell Mcf-7 using DNA microarray. [Proceedings Paper]

The study was carried out to compare the gene expression pattern between Nipah virus NiV infected cells and uninfected cells using DNA microarray technology. MCF-7 cells human metastatic mammary carcinoma cell line were chosen as a host cell for NiV infection. The infection of NiV was successful as the cytophatic effect CPE was observed with the formation of ;multinucleated; giant cells in the infected cell line. Total RNA was isolated from treated and normal MCF-7 cells at 60 hours post infection when the CPE was 80 completed. Microarray analysis was then performed using the total RNA from both cells. The differential expression analysis results showed that 180 genes were down-regulated and 490 genes were up-regulated during NiV infection of MCF-7.

Full text available from: Official URL: https://docs.google.com/file/d/0B7urwQoldOjeaW5kTm...

Abstract

The study was carried out to compare the gene expression pattern between Nipah virus NiV infected cells and uninfected cells using DNA microarray technology. MCF-7 cells human metastatic mammary carcinoma cell line were chosen as a host cell for NiV infection. The infection of NiV was successful as the cytophatic effect CPE was observed with the formation of ;multinucleated; giant cells in the infected cell line. Total RNA was isolated from treated and normal MCF-7 cells at 60 hours post infection when the CPE was 80 completed. Microarray analysis was then performed using the total RNA from both cells. The differential expression analysis results showed that 180 genes were down-regulated and 490 genes were up-regulated during NiV infection of MCF-7.

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Item Type:	Proceedings Paper
AGROVOC Term:	Paramyxoviridae
AGROVOC Term:	Pathogenic viruses
AGROVOC Term:	Biological techniques in vitro
AGROVOC Term:	Gene expression
AGROVOC Term:	Infection
AGROVOC Term:	Genes
Geographical Term:	MALAYSIA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 05:16
URI:	http://webagris.upm.edu.my/id/eprint/13816

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