Citation
Setiawati Umi, . and Mutholib Abdul, . and Forster Brian P., . and Nelson Stephen, . and Nasution Olviyani, . and Caligari Peter, . and Sitorus Andrew, . (2011) Effects of extra-cellular borate and sucrose on in vitro germination of pollen from oil palm of different ploidy levels. [Proceedings Paper]
Abstract
Mature pollen of oil palm is bi-nucleate with one vegetative and one generative cell. During pollen germination and pollen tube growth both nuclei move into the pollen tube where the generative nucleus divides to produce two sperm cells. Boron is an essential micronutrient involved in pollen tube growth. The effects of boron were studied in in vitro germination of pollen produced from oil palms of differing ploidy levels. Boron was applied as boric acid to a liquid pollen germination medium at concentrations ranging from O to 80 mM. Cytological examination of pollen germination and pollen tube growth were performed after 3 hours of adding mature pollen to the culture medium. Three different assessment methods were used: 1 vital staining FDA; 2 DNA staining using fluorescence detection from DAPI; and 3 aceto-orcein staining of nuclei. In the absence of borate pollen from diploid and triploid oil palm exhibited low germination rates which was associated with low fertility. This contrasted with a high rate in the presence of 1.6 mM boric acid. Borate concentrations failed to have an impact on pollen from haploid oil palm. The addition of borate to the germination medium was found to be beneficial and increased pollen fertility by up to 15 and provided optimal conditions for measuring pollen fertility in oil palm.
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Abstract
Mature pollen of oil palm is bi-nucleate with one vegetative and one generative cell. During pollen germination and pollen tube growth both nuclei move into the pollen tube where the generative nucleus divides to produce two sperm cells. Boron is an essential micronutrient involved in pollen tube growth. The effects of boron were studied in in vitro germination of pollen produced from oil palms of differing ploidy levels. Boron was applied as boric acid to a liquid pollen germination medium at concentrations ranging from O to 80 mM. Cytological examination of pollen germination and pollen tube growth were performed after 3 hours of adding mature pollen to the culture medium. Three different assessment methods were used: 1 vital staining FDA; 2 DNA staining using fluorescence detection from DAPI; and 3 aceto-orcein staining of nuclei. In the absence of borate pollen from diploid and triploid oil palm exhibited low germination rates which was associated with low fertility. This contrasted with a high rate in the presence of 1.6 mM boric acid. Borate concentrations failed to have an impact on pollen from haploid oil palm. The addition of borate to the germination medium was found to be beneficial and increased pollen fertility by up to 15 and provided optimal conditions for measuring pollen fertility in oil palm.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP684 P3I61 2011 Call Number |
| AGROVOC Term: | Oil palm |
| AGROVOC Term: | Pollen germination |
| AGROVOC Term: | Sucrose |
| AGROVOC Term: | Plant production |
| AGROVOC Term: | Pollen culture |
| AGROVOC Term: | Experiments |
| AGROVOC Term: | Food supplementation |
| AGROVOC Term: | Cropping patterns |
| AGROVOC Term: | Reproduction |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 05:16 |
| URI: | http://webagris.upm.edu.my/id/eprint/13843 |
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