Tissue culture of some rattan species

Umali-Garcia M., . (1985) Tissue culture of some rattan species. [Proceedings Paper]

An attempt to propagate rattan by tissue culture was conducted using 11 species of Calamus and 2 species of Daemonorops. Preliminary results showed that all the species tested could produce callus from the cabbage or tissues prepared from the youngest portion of the shoot apex on the different media used. The calli remained active for 3-4 weeks under the growing condition described. Callus tissue of Calamus merrillii C. ornatus var. philippinensis and Calamus sp. was able to produce shoots in media supplied with 2-4 sucrose in the presence of 1.0 mg/L benzyl adenine and 1.0 mg/L 2 4-D. More roots were formed when the shoots were transferred to a medium with 4.0 of sucrose and only traces of auxin and cytokinin

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Abstract

An attempt to propagate rattan by tissue culture was conducted using 11 species of Calamus and 2 species of Daemonorops. Preliminary results showed that all the species tested could produce callus from the cabbage or tissues prepared from the youngest portion of the shoot apex on the different media used. The calli remained active for 3-4 weeks under the growing condition described. Callus tissue of Calamus merrillii C. ornatus var. philippinensis and Calamus sp. was able to produce shoots in media supplied with 2-4 sucrose in the presence of 1.0 mg/L benzyl adenine and 1.0 mg/L 2 4-D. More roots were formed when the shoots were transferred to a medium with 4.0 of sucrose and only traces of auxin and cytokinin

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Item Type:	Proceedings Paper
Additional Information:	13 ref. Summary En. Appendix
AGROVOC Term:	RATAN
AGROVOC Term:	CULTIVO DE TEJIDOS
AGROVOC Term:	PROPAGACION DE PLANTAS
AGROVOC Term:	FILIPINAS
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:25
URI:	http://webagris.upm.edu.my/id/eprint/14126

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