Citation
Low Y.L., . and Othman Omar, . and Jalil Abd Kader, . (1990) Synergism activities of the combinations of cellobiohydrolases from Aspergillus terreus SUK-1. [Proceedings Paper]
Abstract
At least three fractions of cellobiohydrolases FPases EC 3.2.1.91 were partially purified from the culture filtrate of Aspergillus terreus SUK-1 through Ammonium Sulphate precipitation gel filtration chromatography Sephadex G150 and anion exchange chromatography DEAE-Sephadex A25. The three enzymes preparation namely as FPase I II and III were shown to be nearly homogenous on the 7.5 native polyacrylamide gel. There is no significant synergism observed between FPase and CMCase. However the activities of the combinations of FPase I - FPase II and FPase II - FPase III were synergistic to any substrate used in the assay. No definite ratio among the enzymes for maximal synergism could be established. The activities with FPase I-FPase III showed no synergism and the substrates used in order of high degree of synergism were carboxymethyl cellulose newspring filter paper and a-cellulose.
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Abstract
At least three fractions of cellobiohydrolases FPases EC 3.2.1.91 were partially purified from the culture filtrate of Aspergillus terreus SUK-1 through Ammonium Sulphate precipitation gel filtration chromatography Sephadex G150 and anion exchange chromatography DEAE-Sephadex A25. The three enzymes preparation namely as FPase I II and III were shown to be nearly homogenous on the 7.5 native polyacrylamide gel. There is no significant synergism observed between FPase and CMCase. However the activities of the combinations of FPase I - FPase II and FPase II - FPase III were synergistic to any substrate used in the assay. No definite ratio among the enzymes for maximal synergism could be established. The activities with FPase I-FPase III showed no synergism and the substrates used in order of high degree of synergism were carboxymethyl cellulose newspring filter paper and a-cellulose.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | 5 graphs; 1 table; 11 ref. Summary En |
| AGROVOC Term: | CELULASA |
| AGROVOC Term: | ASPERGILLUS TERREUS |
| AGROVOC Term: | CROMATOGRAFIA DE CAMBIO IONICO |
| AGROVOC Term: | CROMATOGRAFIA SOBRE GEL/ SULFATO DE AMONIO |
| AGROVOC Term: | SINERGISMO |
| AGROVOC Term: | ACTIVIDAD ENZIMATICA |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:26 |
| URI: | http://webagris.upm.edu.my/id/eprint/14741 |
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