An inoculation technique to assess soybean plants for response to bacterial pustule

Hardaningsih S., . and Cook A.A., . (1990) An inoculation technique to assess soybean plants for response to bacterial pustule. [Proceedings Paper]

Bacterial pustule of soybean caused by Xanthomonas campestris pv glycines is a disease of major significance in Indonesia. Several exotic soybean cultivars are considered resistant but we want a technique to qualitatively assess these and other cultivars for response to the Indonesian pathogen. While various techniques have been used to pustule infection in soybean none of these techniques permit accurate qualitative assessment of individual plant reponse. We have found fully expanded cotyledons of plants one week from seeding can be easily infiltrated by hypodermic injection. Injection of 1x104 bacterial cfu causes cotyledons of susceptible plants to turn cholorotic-necrotic and the fall within one week of inoculation while cotyledons on resistant plants remain green and firmly attached. Bacterial population in vivo were found to be 250X greater in susceptible than in resistant cotyledons 144 hrs after incubation. This technique can be used to characterize cultivars as well as to select indivual plants of known response while they are still young enough for additional disease testing or for use in a breeding programme

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Abstract

Bacterial pustule of soybean caused by Xanthomonas campestris pv glycines is a disease of major significance in Indonesia. Several exotic soybean cultivars are considered resistant but we want a technique to qualitatively assess these and other cultivars for response to the Indonesian pathogen. While various techniques have been used to pustule infection in soybean none of these techniques permit accurate qualitative assessment of individual plant reponse. We have found fully expanded cotyledons of plants one week from seeding can be easily infiltrated by hypodermic injection. Injection of 1x104 bacterial cfu causes cotyledons of susceptible plants to turn cholorotic-necrotic and the fall within one week of inoculation while cotyledons on resistant plants remain green and firmly attached. Bacterial population in vivo were found to be 250X greater in susceptible than in resistant cotyledons 144 hrs after incubation. This technique can be used to characterize cultivars as well as to select indivual plants of known response while they are still young enough for additional disease testing or for use in a breeding programme

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Item Type:	Proceedings Paper
Additional Information:	5 refs.; Summary En
AGROVOC Term:	SOJA
AGROVOC Term:	XANTHOMONAS CAMPESTRIS
AGROVOC Term:	ENFERMEDADES DE LAS PLANTAS
AGROVOC Term:	INOCULACION
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:26
URI:	http://webagris.upm.edu.my/id/eprint/14998

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